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An integrated workflow for charting the human interaction proteome: insights into the PP2A system
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS an...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2644174/ https://www.ncbi.nlm.nih.gov/pubmed/19156129 http://dx.doi.org/10.1038/msb.2008.75 |
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author | Glatter, Timo Wepf, Alexander Aebersold, Ruedi Gstaiger, Matthias |
author_facet | Glatter, Timo Wepf, Alexander Aebersold, Ruedi Gstaiger, Matthias |
author_sort | Glatter, Timo |
collection | PubMed |
description | Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP-MS studies on human protein complexes. |
format | Text |
id | pubmed-2644174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-26441742009-02-19 An integrated workflow for charting the human interaction proteome: insights into the PP2A system Glatter, Timo Wepf, Alexander Aebersold, Ruedi Gstaiger, Matthias Mol Syst Biol Article Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP-MS studies on human protein complexes. Nature Publishing Group 2009-01-20 /pmc/articles/PMC2644174/ /pubmed/19156129 http://dx.doi.org/10.1038/msb.2008.75 Text en Copyright © 2009, EMBO and Nature Publishing Group http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits distribution and reproduction in any medium, provided the original author and source are credited. Creation of derivative works is permitted but the resulting work may be distributed only under the same or similar licence to this one. This licence does not permit commercial exploitation without specific permission. |
spellingShingle | Article Glatter, Timo Wepf, Alexander Aebersold, Ruedi Gstaiger, Matthias An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title_full | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title_fullStr | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title_full_unstemmed | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title_short | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
title_sort | integrated workflow for charting the human interaction proteome: insights into the pp2a system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2644174/ https://www.ncbi.nlm.nih.gov/pubmed/19156129 http://dx.doi.org/10.1038/msb.2008.75 |
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