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Optimal use of tandem biotin and V5 tags in ChIP assays

BACKGROUND: Chromatin immunoprecipitation (ChIP) assays coupled to genome arrays (Chip-on-chip) or massive parallel sequencing (ChIP-seq) lead to the genome wide identification of binding sites of chromatin associated proteins. However, the highly variable quality of antibodies and the availability...

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Detalles Bibliográficos
Autores principales: Kolodziej, Katarzyna E, Pourfarzad, Farzin, de Boer, Ernie, Krpic, Sanja, Grosveld, Frank, Strouboulis, John
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2644297/
https://www.ncbi.nlm.nih.gov/pubmed/19196479
http://dx.doi.org/10.1186/1471-2199-10-6