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Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function
CD4(+) recent thymic emigrants (RTEs) comprise a clinically and immunologically important T cell population that indicates thymic output and that is essential for maintaining a diverse αβ–T cell receptor (TCR) repertoire of the naive CD4(+) T cell compartment. However, their frequency and function a...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646563/ https://www.ncbi.nlm.nih.gov/pubmed/19171767 http://dx.doi.org/10.1084/jem.20080996 |
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author | Haines, Christopher J. Giffon, Thierry D. Lu, Li-Sheng Lu, Xiaowei Tessier-Lavigne, Marc Ross, Douglas T. Lewis, David B. |
author_facet | Haines, Christopher J. Giffon, Thierry D. Lu, Li-Sheng Lu, Xiaowei Tessier-Lavigne, Marc Ross, Douglas T. Lewis, David B. |
author_sort | Haines, Christopher J. |
collection | PubMed |
description | CD4(+) recent thymic emigrants (RTEs) comprise a clinically and immunologically important T cell population that indicates thymic output and that is essential for maintaining a diverse αβ–T cell receptor (TCR) repertoire of the naive CD4(+) T cell compartment. However, their frequency and function are poorly understood because no known surface markers distinguish them from older non-RTE naive CD4(+) T cells. We demonstrate that protein tyrosine kinase 7 (PTK7) is a novel marker for human CD4(+) RTEs. Consistent with their recent thymic origin, human PTK7(+) RTEs contained higher levels of signal joint TCR gene excision circles and were more responsive to interleukin (IL)-7 compared with PTK7(−) naive CD4(+) T cells, and rapidly decreased after complete thymectomy. Importantly, CD4(+) RTEs proliferated less and produced less IL-2 and interferon-γ than PTK7(−) naive CD4(+) T cells after αβ-TCR/CD3 and CD28 engagement. This immaturity in CD4(+) RTE effector function may contribute to the reduced CD4(+) T cell immunity observed in contexts in which CD4(+) RTEs predominate, such as in the fetus and neonate or after immune reconstitution. The ability to identify viable CD4(+) RTEs by PTK7 staining should be useful for monitoring thymic output in both healthy individuals and in patients with genetic or acquired CD4(+) T cell immunodeficiencies. |
format | Text |
id | pubmed-2646563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26465632009-08-16 Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function Haines, Christopher J. Giffon, Thierry D. Lu, Li-Sheng Lu, Xiaowei Tessier-Lavigne, Marc Ross, Douglas T. Lewis, David B. J Exp Med Brief Definitive Report CD4(+) recent thymic emigrants (RTEs) comprise a clinically and immunologically important T cell population that indicates thymic output and that is essential for maintaining a diverse αβ–T cell receptor (TCR) repertoire of the naive CD4(+) T cell compartment. However, their frequency and function are poorly understood because no known surface markers distinguish them from older non-RTE naive CD4(+) T cells. We demonstrate that protein tyrosine kinase 7 (PTK7) is a novel marker for human CD4(+) RTEs. Consistent with their recent thymic origin, human PTK7(+) RTEs contained higher levels of signal joint TCR gene excision circles and were more responsive to interleukin (IL)-7 compared with PTK7(−) naive CD4(+) T cells, and rapidly decreased after complete thymectomy. Importantly, CD4(+) RTEs proliferated less and produced less IL-2 and interferon-γ than PTK7(−) naive CD4(+) T cells after αβ-TCR/CD3 and CD28 engagement. This immaturity in CD4(+) RTE effector function may contribute to the reduced CD4(+) T cell immunity observed in contexts in which CD4(+) RTEs predominate, such as in the fetus and neonate or after immune reconstitution. The ability to identify viable CD4(+) RTEs by PTK7 staining should be useful for monitoring thymic output in both healthy individuals and in patients with genetic or acquired CD4(+) T cell immunodeficiencies. The Rockefeller University Press 2009-02-16 /pmc/articles/PMC2646563/ /pubmed/19171767 http://dx.doi.org/10.1084/jem.20080996 Text en © 2009 Haines et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Brief Definitive Report Haines, Christopher J. Giffon, Thierry D. Lu, Li-Sheng Lu, Xiaowei Tessier-Lavigne, Marc Ross, Douglas T. Lewis, David B. Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title | Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title_full | Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title_fullStr | Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title_full_unstemmed | Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title_short | Human CD4(+) T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
title_sort | human cd4(+) t cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646563/ https://www.ncbi.nlm.nih.gov/pubmed/19171767 http://dx.doi.org/10.1084/jem.20080996 |
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