Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments

[Image: see text] Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a...

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Autores principales: Dudkin, Vadim Y., Miller, Justin S., Dudkina, Anna S., Antczak, Christophe, Scheinberg, David A., Danishefsky, Samuel J.
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2008
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646745/
https://www.ncbi.nlm.nih.gov/pubmed/18798614
http://dx.doi.org/10.1021/ja8028137
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author Dudkin, Vadim Y.
Miller, Justin S.
Dudkina, Anna S.
Antczak, Christophe
Scheinberg, David A.
Danishefsky, Samuel J.
author_facet Dudkin, Vadim Y.
Miller, Justin S.
Dudkina, Anna S.
Antczak, Christophe
Scheinberg, David A.
Danishefsky, Samuel J.
author_sort Dudkin, Vadim Y.
collection PubMed
description [Image: see text] Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation.
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spelling pubmed-26467452009-03-20 Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments Dudkin, Vadim Y. Miller, Justin S. Dudkina, Anna S. Antczak, Christophe Scheinberg, David A. Danishefsky, Samuel J. J Am Chem Soc [Image: see text] Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation. American Chemical Society 2008-09-18 2008-10-15 /pmc/articles/PMC2646745/ /pubmed/18798614 http://dx.doi.org/10.1021/ja8028137 Text en Copyright © 2008 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org. 40.75
spellingShingle Dudkin, Vadim Y.
Miller, Justin S.
Dudkina, Anna S.
Antczak, Christophe
Scheinberg, David A.
Danishefsky, Samuel J.
Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title_full Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title_fullStr Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title_full_unstemmed Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title_short Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments
title_sort toward a prostate specific antigen-based prostate cancer diagnostic assay: preparation of keyhole limpet hemocyanin -conjugated normal and transformed prostate specific antigen fragments
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646745/
https://www.ncbi.nlm.nih.gov/pubmed/18798614
http://dx.doi.org/10.1021/ja8028137
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