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Altered microRNA expression in patients with non-obstructive azoospermia
BACKGROUND: MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility. METHODS: In this study, the miRNA expression prof...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647923/ https://www.ncbi.nlm.nih.gov/pubmed/19210773 http://dx.doi.org/10.1186/1477-7827-7-13 |
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author | Lian, Jie Zhang, Xiansheng Tian, Hui Liang, Ning Wang, Yong Liang, Chaozhao Li, Xin Sun, Fei |
author_facet | Lian, Jie Zhang, Xiansheng Tian, Hui Liang, Ning Wang, Yong Liang, Chaozhao Li, Xin Sun, Fei |
author_sort | Lian, Jie |
collection | PubMed |
description | BACKGROUND: MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility. METHODS: In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies. RESULTS: Altered microRNA expression in NOA patients was found, with 154 differentially down-regulated and 19 up-regulated miRNAs. These findings have been confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) assays on select miRNAs, including miR-302a, miR-491-3p, miR-520d-3p and miR-383. Several down-regulated miRNA clusters in patients with NOA were identified, such as the oncogenic potential of the mir-17-92 cluster and mir-371,2,3 cluster. CONCLUSION: This is the first report that the expression of miRNAs is altered in testicular tissues of patients with NOA, suggesting a role of miRNAs in regulating spermatogenesis in human males. |
format | Text |
id | pubmed-2647923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26479232009-02-26 Altered microRNA expression in patients with non-obstructive azoospermia Lian, Jie Zhang, Xiansheng Tian, Hui Liang, Ning Wang, Yong Liang, Chaozhao Li, Xin Sun, Fei Reprod Biol Endocrinol Research BACKGROUND: MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility. METHODS: In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies. RESULTS: Altered microRNA expression in NOA patients was found, with 154 differentially down-regulated and 19 up-regulated miRNAs. These findings have been confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) assays on select miRNAs, including miR-302a, miR-491-3p, miR-520d-3p and miR-383. Several down-regulated miRNA clusters in patients with NOA were identified, such as the oncogenic potential of the mir-17-92 cluster and mir-371,2,3 cluster. CONCLUSION: This is the first report that the expression of miRNAs is altered in testicular tissues of patients with NOA, suggesting a role of miRNAs in regulating spermatogenesis in human males. BioMed Central 2009-02-11 /pmc/articles/PMC2647923/ /pubmed/19210773 http://dx.doi.org/10.1186/1477-7827-7-13 Text en Copyright © 2009 Lian et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Lian, Jie Zhang, Xiansheng Tian, Hui Liang, Ning Wang, Yong Liang, Chaozhao Li, Xin Sun, Fei Altered microRNA expression in patients with non-obstructive azoospermia |
title | Altered microRNA expression in patients with non-obstructive azoospermia |
title_full | Altered microRNA expression in patients with non-obstructive azoospermia |
title_fullStr | Altered microRNA expression in patients with non-obstructive azoospermia |
title_full_unstemmed | Altered microRNA expression in patients with non-obstructive azoospermia |
title_short | Altered microRNA expression in patients with non-obstructive azoospermia |
title_sort | altered microrna expression in patients with non-obstructive azoospermia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647923/ https://www.ncbi.nlm.nih.gov/pubmed/19210773 http://dx.doi.org/10.1186/1477-7827-7-13 |
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