Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China

In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on...

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Autores principales: Zhang, Wen, Yang, Shixing, Shen, Quan, Liu, Junfeng, Shan, Tongling, Huang, Fen, Ning, Huibo, Kang, Yanjun, Yang, Zhibiao, Cui, Li, Zhu, Jianguo, Hua, Xiuguo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649060/
https://www.ncbi.nlm.nih.gov/pubmed/19220877
http://dx.doi.org/10.1186/1743-422X-6-24
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author Zhang, Wen
Yang, Shixing
Shen, Quan
Liu, Junfeng
Shan, Tongling
Huang, Fen
Ning, Huibo
Kang, Yanjun
Yang, Zhibiao
Cui, Li
Zhu, Jianguo
Hua, Xiuguo
author_facet Zhang, Wen
Yang, Shixing
Shen, Quan
Liu, Junfeng
Shan, Tongling
Huang, Fen
Ning, Huibo
Kang, Yanjun
Yang, Zhibiao
Cui, Li
Zhu, Jianguo
Hua, Xiuguo
author_sort Zhang, Wen
collection PubMed
description In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on the full ORF2 sequence indicated that this HEV strain shared the highest sequence identity (97.6%) with another human HEV strain isolated from a Japanese patient who was infected by genotype 4 HEV during traveling in Shanghai. Phylogenetic analysis showed that this genotype 4 HEV was phylogenetically far from the genotype 4 HEV strain that was commonly prevalent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in this area.
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spelling pubmed-26490602009-02-28 Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China Zhang, Wen Yang, Shixing Shen, Quan Liu, Junfeng Shan, Tongling Huang, Fen Ning, Huibo Kang, Yanjun Yang, Zhibiao Cui, Li Zhu, Jianguo Hua, Xiuguo Virol J Short Report In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on the full ORF2 sequence indicated that this HEV strain shared the highest sequence identity (97.6%) with another human HEV strain isolated from a Japanese patient who was infected by genotype 4 HEV during traveling in Shanghai. Phylogenetic analysis showed that this genotype 4 HEV was phylogenetically far from the genotype 4 HEV strain that was commonly prevalent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in this area. BioMed Central 2009-02-16 /pmc/articles/PMC2649060/ /pubmed/19220877 http://dx.doi.org/10.1186/1743-422X-6-24 Text en Copyright © 2009 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Zhang, Wen
Yang, Shixing
Shen, Quan
Liu, Junfeng
Shan, Tongling
Huang, Fen
Ning, Huibo
Kang, Yanjun
Yang, Zhibiao
Cui, Li
Zhu, Jianguo
Hua, Xiuguo
Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title_full Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title_fullStr Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title_full_unstemmed Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title_short Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China
title_sort isolation and characterization of a genotype 4 hepatitis e virus strain from an infant in china
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649060/
https://www.ncbi.nlm.nih.gov/pubmed/19220877
http://dx.doi.org/10.1186/1743-422X-6-24
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