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A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana
BACKGROUND: Diapause is a reversible state of developmental suspension and found among diverse taxa, from plants to animals, including marsupials and some other mammals. Although previous work has accumulated ample data, the molecular mechanism underlying diapause and reactivation from it remain elu...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649162/ https://www.ncbi.nlm.nih.gov/pubmed/19173719 http://dx.doi.org/10.1186/1471-2164-10-52 |
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author | Chen, Wie-Hua Ge, Xiaomeng Wang, Weiwei Yu, Jun Hu, Songnian |
author_facet | Chen, Wie-Hua Ge, Xiaomeng Wang, Weiwei Yu, Jun Hu, Songnian |
author_sort | Chen, Wie-Hua |
collection | PubMed |
description | BACKGROUND: Diapause is a reversible state of developmental suspension and found among diverse taxa, from plants to animals, including marsupials and some other mammals. Although previous work has accumulated ample data, the molecular mechanism underlying diapause and reactivation from it remain elusive. RESULTS: Using Artemia franciscana, a model organism to study the development of post-diapause embryos in Arthropod, we sequenced random clones up to a total of 28,039 ESTs from four cDNA libraries made from dehydrated cysts and three time points after rehydration/reactivation, which were assembled into 8,018 unigene clusters. We identified 324 differentially-expressed genes (DEGs, P < 0.05) based on pairwise comparisons of the four cDNA libraries. We identified a group of genes that are involved in an anti-water-deficit system, including proteases, protease inhibitors, heat shock proteins, and several novel members of the late embryogenesis abundant (LEA) protein family. In addition, we classified most of the up-regulated genes after cyst reactivation into metabolism, biosynthesis, transcription, and translation, and this result is consistent with the rapid development of the embryo. Some of the specific expressions of DEGs were confirmed experimentally based on quantitative real-time PCR. CONCLUSION: We found that the first 5-hour period after rehydration is most important for embryonic reactivation of Artemia. As the total number of expressed genes increases significantly, the majority of DEGs were also identified in this period, including a group of water-deficient-induced genes. A group of genes with similar functions have been described in plant seeds; for instance, one of the novel LEA members shares ~70% amino-acid identity with an Arabidopsis EM (embryonic abundant) protein, the closest animal relative to plant LEA families identified thus far. Our findings also suggested that not only nutrition, but also mRNAs are produced and stored during cyst formation to support rapid development after reactivation. |
format | Text |
id | pubmed-2649162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26491622009-02-28 A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana Chen, Wie-Hua Ge, Xiaomeng Wang, Weiwei Yu, Jun Hu, Songnian BMC Genomics Research Article BACKGROUND: Diapause is a reversible state of developmental suspension and found among diverse taxa, from plants to animals, including marsupials and some other mammals. Although previous work has accumulated ample data, the molecular mechanism underlying diapause and reactivation from it remain elusive. RESULTS: Using Artemia franciscana, a model organism to study the development of post-diapause embryos in Arthropod, we sequenced random clones up to a total of 28,039 ESTs from four cDNA libraries made from dehydrated cysts and three time points after rehydration/reactivation, which were assembled into 8,018 unigene clusters. We identified 324 differentially-expressed genes (DEGs, P < 0.05) based on pairwise comparisons of the four cDNA libraries. We identified a group of genes that are involved in an anti-water-deficit system, including proteases, protease inhibitors, heat shock proteins, and several novel members of the late embryogenesis abundant (LEA) protein family. In addition, we classified most of the up-regulated genes after cyst reactivation into metabolism, biosynthesis, transcription, and translation, and this result is consistent with the rapid development of the embryo. Some of the specific expressions of DEGs were confirmed experimentally based on quantitative real-time PCR. CONCLUSION: We found that the first 5-hour period after rehydration is most important for embryonic reactivation of Artemia. As the total number of expressed genes increases significantly, the majority of DEGs were also identified in this period, including a group of water-deficient-induced genes. A group of genes with similar functions have been described in plant seeds; for instance, one of the novel LEA members shares ~70% amino-acid identity with an Arabidopsis EM (embryonic abundant) protein, the closest animal relative to plant LEA families identified thus far. Our findings also suggested that not only nutrition, but also mRNAs are produced and stored during cyst formation to support rapid development after reactivation. BioMed Central 2009-01-27 /pmc/articles/PMC2649162/ /pubmed/19173719 http://dx.doi.org/10.1186/1471-2164-10-52 Text en Copyright © 2009 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Wie-Hua Ge, Xiaomeng Wang, Weiwei Yu, Jun Hu, Songnian A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title | A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title_full | A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title_fullStr | A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title_full_unstemmed | A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title_short | A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana |
title_sort | gene catalogue for post-diapause development of an anhydrobiotic arthropod artemia franciscana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649162/ https://www.ncbi.nlm.nih.gov/pubmed/19173719 http://dx.doi.org/10.1186/1471-2164-10-52 |
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