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Identification of Differentially Expressed Genes in Papillary Thyroid Cancers
PURPOSE: Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique™ using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Yonsei University College of Medicine
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649849/ https://www.ncbi.nlm.nih.gov/pubmed/19259350 http://dx.doi.org/10.3349/ymj.2009.50.1.60 |
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author | Lee, Ki-Young Huang, Song Mei Li, Shengjin Kim, Jin-Man |
author_facet | Lee, Ki-Young Huang, Song Mei Li, Shengjin Kim, Jin-Man |
author_sort | Lee, Ki-Young |
collection | PubMed |
description | PURPOSE: Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique™ using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid cancer (PTC) by using Genefishing technique™. MATERIALS AND METHODS: We utilized a new differential display method, designated with Genefishing technique™, to analyze DEGs in 21 cases of PTCs. RESULTS: Comparing the gene expression profiles between PTC and normal thyroid, we detected 17 genes that were differentially expressed in PTCs and performed cloning with sequencing in 10 genes. We confirmed the expression patterns of 2 DEGs by RT-PCR assay and identified the same results in 17 out of 21 (81%) PTCs. The 2 DEGs over-expressed in PTCs were identified as DC-STAMP and type I collagen A1. They are novel genes identified first in PTCs. CONCLUSION: We confirmed 2 DEGs in PTCs as DC-STAMP and type I collagen A1 by using Genefishing technique™. Although the detailed functions of those 2 genes and their products remain to be determined, the genes will provide insights into mechanisms of carcinogenesis or tumor progression in PTCs. |
format | Text |
id | pubmed-2649849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Yonsei University College of Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-26498492009-03-03 Identification of Differentially Expressed Genes in Papillary Thyroid Cancers Lee, Ki-Young Huang, Song Mei Li, Shengjin Kim, Jin-Man Yonsei Med J Original Article PURPOSE: Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique™ using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid cancer (PTC) by using Genefishing technique™. MATERIALS AND METHODS: We utilized a new differential display method, designated with Genefishing technique™, to analyze DEGs in 21 cases of PTCs. RESULTS: Comparing the gene expression profiles between PTC and normal thyroid, we detected 17 genes that were differentially expressed in PTCs and performed cloning with sequencing in 10 genes. We confirmed the expression patterns of 2 DEGs by RT-PCR assay and identified the same results in 17 out of 21 (81%) PTCs. The 2 DEGs over-expressed in PTCs were identified as DC-STAMP and type I collagen A1. They are novel genes identified first in PTCs. CONCLUSION: We confirmed 2 DEGs in PTCs as DC-STAMP and type I collagen A1 by using Genefishing technique™. Although the detailed functions of those 2 genes and their products remain to be determined, the genes will provide insights into mechanisms of carcinogenesis or tumor progression in PTCs. Yonsei University College of Medicine 2009-02-28 2009-02-24 /pmc/articles/PMC2649849/ /pubmed/19259350 http://dx.doi.org/10.3349/ymj.2009.50.1.60 Text en Copyright © 2009 The Yonsei University College of Medicine http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Ki-Young Huang, Song Mei Li, Shengjin Kim, Jin-Man Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title | Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title_full | Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title_fullStr | Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title_full_unstemmed | Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title_short | Identification of Differentially Expressed Genes in Papillary Thyroid Cancers |
title_sort | identification of differentially expressed genes in papillary thyroid cancers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649849/ https://www.ncbi.nlm.nih.gov/pubmed/19259350 http://dx.doi.org/10.3349/ymj.2009.50.1.60 |
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