A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

BACKGROUND: Many protocols for recombinant production of peptides and proteins include secretion into the periplasmic space of Escherichia coli, as they may not properly fold in the cytoplasm. If a signal peptide is not sufficient for translocation, a larger secretion moiety can instead be fused to...

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Detalles Bibliográficos
Autores principales: Paal, Michael, Heel, Thomas, Schneider, Rainer, Auer, Bernhard
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649888/
https://www.ncbi.nlm.nih.gov/pubmed/19159462
http://dx.doi.org/10.1186/1475-2859-8-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649888/
https://www.ncbi.nlm.nih.gov/pubmed/19159462
http://dx.doi.org/10.1186/1475-2859-8-7

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