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Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy

Electroconvulsive therapy (ECT) is one of the most effective treatments used in psychiatry to date. The mechanisms of ECT action, however, are the least understood and still unclear. As a tool to elucidate the mechanisms of action of ECT, we employed proteomic analysis based on the identification of...

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Autores principales: Lee, Cheol Soon, Kang, Kee Ryeon, Lee, Ji-Young, Park, Chul Soo, Hahn, Kyu Hee, Sohn, Jin Wook, Kim, Bong Jo
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650984/
https://www.ncbi.nlm.nih.gov/pubmed/19270826
http://dx.doi.org/10.3346/jkms.2009.24.1.132
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author Lee, Cheol Soon
Kang, Kee Ryeon
Lee, Ji-Young
Park, Chul Soo
Hahn, Kyu Hee
Sohn, Jin Wook
Kim, Bong Jo
author_facet Lee, Cheol Soon
Kang, Kee Ryeon
Lee, Ji-Young
Park, Chul Soo
Hahn, Kyu Hee
Sohn, Jin Wook
Kim, Bong Jo
author_sort Lee, Cheol Soon
collection PubMed
description Electroconvulsive therapy (ECT) is one of the most effective treatments used in psychiatry to date. The mechanisms of ECT action, however, are the least understood and still unclear. As a tool to elucidate the mechanisms of action of ECT, we employed proteomic analysis based on the identification of differentially expressed proteins after exposure to repeated ECT in rat brains. The expression of proteins was visualized by silver stain after two-dimensional gel electrophoresis. Of 24 differentially expressed protein spots (p<0.05 by Student t-test), six different proteins from 7 spots were identified by matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF)/mass spectrometry. Among the identified proteins, there were five dominantly expressed proteins in the ECT-treated rat brain tissues (p<0.05); S100 protein beta chain, 14-3-3 protein zeta/delta, similar to ubiquitin-like 1 (sentrin) activating enzyme subunit 1, suppressor of G2 allele of SKP1 homolog, and phosphatidylinositol transfer protein alpha. The expression of only one protein, ACY1 protein, was repressed (p<0.05). These findings likely serve for a better understanding of mechanisms involved in the therapeutic effects of ECT.
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spelling pubmed-26509842009-03-06 Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy Lee, Cheol Soon Kang, Kee Ryeon Lee, Ji-Young Park, Chul Soo Hahn, Kyu Hee Sohn, Jin Wook Kim, Bong Jo J Korean Med Sci Original Article Electroconvulsive therapy (ECT) is one of the most effective treatments used in psychiatry to date. The mechanisms of ECT action, however, are the least understood and still unclear. As a tool to elucidate the mechanisms of action of ECT, we employed proteomic analysis based on the identification of differentially expressed proteins after exposure to repeated ECT in rat brains. The expression of proteins was visualized by silver stain after two-dimensional gel electrophoresis. Of 24 differentially expressed protein spots (p<0.05 by Student t-test), six different proteins from 7 spots were identified by matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF)/mass spectrometry. Among the identified proteins, there were five dominantly expressed proteins in the ECT-treated rat brain tissues (p<0.05); S100 protein beta chain, 14-3-3 protein zeta/delta, similar to ubiquitin-like 1 (sentrin) activating enzyme subunit 1, suppressor of G2 allele of SKP1 homolog, and phosphatidylinositol transfer protein alpha. The expression of only one protein, ACY1 protein, was repressed (p<0.05). These findings likely serve for a better understanding of mechanisms involved in the therapeutic effects of ECT. The Korean Academy of Medical Sciences 2009-02 2009-02-28 /pmc/articles/PMC2650984/ /pubmed/19270826 http://dx.doi.org/10.3346/jkms.2009.24.1.132 Text en Copyright © 2009 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Cheol Soon
Kang, Kee Ryeon
Lee, Ji-Young
Park, Chul Soo
Hahn, Kyu Hee
Sohn, Jin Wook
Kim, Bong Jo
Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title_full Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title_fullStr Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title_full_unstemmed Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title_short Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
title_sort proteomic analysis of rat brains following exposure to electroconvulsive therapy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650984/
https://www.ncbi.nlm.nih.gov/pubmed/19270826
http://dx.doi.org/10.3346/jkms.2009.24.1.132
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