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DNA modification of live cell surface
We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complemen...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651772/ https://www.ncbi.nlm.nih.gov/pubmed/19158188 http://dx.doi.org/10.1093/nar/gkn1034 |
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author | Borisenko, Grigory G. Zaitseva, Marina A. Chuvilin, Andrey N. Pozmogova, Galina E. |
author_facet | Borisenko, Grigory G. Zaitseva, Marina A. Chuvilin, Andrey N. Pozmogova, Galina E. |
author_sort | Borisenko, Grigory G. |
collection | PubMed |
description | We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complementary sequences at the cell surface. Method permits to control amount of immobilized DNA on the cell surface. All procedures can be completed within minutes and do not alter cell viability. Using this approach we tethered floating myeloid HL-60 cells to adherent A431 epitheliocytes in a sequence specific fashion. Thus, this method allows rapid and simple DNA multicoding of the cell surface and, therefore, opens new opportunities in manipulating with cell–cell interactions. |
format | Text |
id | pubmed-2651772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26517722009-03-13 DNA modification of live cell surface Borisenko, Grigory G. Zaitseva, Marina A. Chuvilin, Andrey N. Pozmogova, Galina E. Nucleic Acids Res Methods Online We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complementary sequences at the cell surface. Method permits to control amount of immobilized DNA on the cell surface. All procedures can be completed within minutes and do not alter cell viability. Using this approach we tethered floating myeloid HL-60 cells to adherent A431 epitheliocytes in a sequence specific fashion. Thus, this method allows rapid and simple DNA multicoding of the cell surface and, therefore, opens new opportunities in manipulating with cell–cell interactions. Oxford University Press 2009-03 2009-01-21 /pmc/articles/PMC2651772/ /pubmed/19158188 http://dx.doi.org/10.1093/nar/gkn1034 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methods Online Borisenko, Grigory G. Zaitseva, Marina A. Chuvilin, Andrey N. Pozmogova, Galina E. DNA modification of live cell surface |
title | DNA modification of live cell surface |
title_full | DNA modification of live cell surface |
title_fullStr | DNA modification of live cell surface |
title_full_unstemmed | DNA modification of live cell surface |
title_short | DNA modification of live cell surface |
title_sort | dna modification of live cell surface |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651772/ https://www.ncbi.nlm.nih.gov/pubmed/19158188 http://dx.doi.org/10.1093/nar/gkn1034 |
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