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Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens

BACKGROUND: Marek's disease (MD) is a T-cell lymphoma of chickens caused by the Marek's disease virus (MDV), an oncogenic avian herpesvirus. MD is a major cause of economic loss to the poultry industry and the most serious and persistent infectious disease concern. A full-sib intercross po...

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Autores principales: Heifetz, Eliyahu M, Fulton, Janet E, O'Sullivan, Neil P, Arthur, James A, Cheng, Hans, Wang, Jing, Soller, Morris, Dekkers, Jack CM
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651900/
https://www.ncbi.nlm.nih.gov/pubmed/19144166
http://dx.doi.org/10.1186/1471-2164-10-20
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author Heifetz, Eliyahu M
Fulton, Janet E
O'Sullivan, Neil P
Arthur, James A
Cheng, Hans
Wang, Jing
Soller, Morris
Dekkers, Jack CM
author_facet Heifetz, Eliyahu M
Fulton, Janet E
O'Sullivan, Neil P
Arthur, James A
Cheng, Hans
Wang, Jing
Soller, Morris
Dekkers, Jack CM
author_sort Heifetz, Eliyahu M
collection PubMed
description BACKGROUND: Marek's disease (MD) is a T-cell lymphoma of chickens caused by the Marek's disease virus (MDV), an oncogenic avian herpesvirus. MD is a major cause of economic loss to the poultry industry and the most serious and persistent infectious disease concern. A full-sib intercross population, consisting of five independent families was generated by crossing and repeated intercrossing of two partially inbred commercial White Leghorn layer lines known to differ in genetic resistance to MD. At the F6 generation, a total of 1615 chicks were produced (98 to 248 per family) and phenotyped for MD resistance measured as survival time in days after challenge with a very virulent plus (vv+) strain of MDV. RESULTS: QTL affecting MD resistance were identified by selective DNA pooling using a panel of 15 SNPs and 217 microsatellite markers. Since MHC blood type (BT) is known to affect MD resistance, a total of 18 independent pool pairs were constructed according to family × BT combination, with some combinations represented twice for technical reasons. Twenty-one QTL regions (QTLR) affecting post-challenge survival time were identified, distributed among 11 chromosomes (GGA1, 2, 3, 4, 5, 8, 9, 15, 18, 26 and Z), with about two-thirds of the MD resistance alleles derived from the more MD resistant parental line. Eight of the QTLR associated with MD resistance, were previously identified in a backcross (BC) mapping study with the same parental lines. Of these, 7 originated from the more resistant line, and one from the less resistant line. CONCLUSION: There was considerable evidence suggesting that MD resistance alleles tend to be recessive. The width of the QTLR for these QTL appeared to be reduced about two-fold in the F6 as compared to that found in the previous BC study. These results provide a firm basis for high-resolution linkage disequilibrium mapping and positional cloning of the resistance genes.
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spelling pubmed-26519002009-03-06 Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens Heifetz, Eliyahu M Fulton, Janet E O'Sullivan, Neil P Arthur, James A Cheng, Hans Wang, Jing Soller, Morris Dekkers, Jack CM BMC Genomics Research Article BACKGROUND: Marek's disease (MD) is a T-cell lymphoma of chickens caused by the Marek's disease virus (MDV), an oncogenic avian herpesvirus. MD is a major cause of economic loss to the poultry industry and the most serious and persistent infectious disease concern. A full-sib intercross population, consisting of five independent families was generated by crossing and repeated intercrossing of two partially inbred commercial White Leghorn layer lines known to differ in genetic resistance to MD. At the F6 generation, a total of 1615 chicks were produced (98 to 248 per family) and phenotyped for MD resistance measured as survival time in days after challenge with a very virulent plus (vv+) strain of MDV. RESULTS: QTL affecting MD resistance were identified by selective DNA pooling using a panel of 15 SNPs and 217 microsatellite markers. Since MHC blood type (BT) is known to affect MD resistance, a total of 18 independent pool pairs were constructed according to family × BT combination, with some combinations represented twice for technical reasons. Twenty-one QTL regions (QTLR) affecting post-challenge survival time were identified, distributed among 11 chromosomes (GGA1, 2, 3, 4, 5, 8, 9, 15, 18, 26 and Z), with about two-thirds of the MD resistance alleles derived from the more MD resistant parental line. Eight of the QTLR associated with MD resistance, were previously identified in a backcross (BC) mapping study with the same parental lines. Of these, 7 originated from the more resistant line, and one from the less resistant line. CONCLUSION: There was considerable evidence suggesting that MD resistance alleles tend to be recessive. The width of the QTLR for these QTL appeared to be reduced about two-fold in the F6 as compared to that found in the previous BC study. These results provide a firm basis for high-resolution linkage disequilibrium mapping and positional cloning of the resistance genes. BioMed Central 2009-01-14 /pmc/articles/PMC2651900/ /pubmed/19144166 http://dx.doi.org/10.1186/1471-2164-10-20 Text en Copyright © 2009 Heifetz et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Heifetz, Eliyahu M
Fulton, Janet E
O'Sullivan, Neil P
Arthur, James A
Cheng, Hans
Wang, Jing
Soller, Morris
Dekkers, Jack CM
Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title_full Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title_fullStr Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title_full_unstemmed Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title_short Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens
title_sort mapping qtl affecting resistance to marek's disease in an f6 advanced intercross population of commercial layer chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651900/
https://www.ncbi.nlm.nih.gov/pubmed/19144166
http://dx.doi.org/10.1186/1471-2164-10-20
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