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Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection

Throughout tomato domestication, a large increase in fruit size was associated with a loss of dry matter and sugar contents. This study aims to dissect the contributions of genetic variation and the physiological processes underlying the relationships between fruit growth and the accumulation of dry...

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Autores principales: Prudent, Marion, Causse, Mathilde, Génard, Michel, Tripodi, Pasquale, Grandillo, Silvana, Bertin, Nadia
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652058/
https://www.ncbi.nlm.nih.gov/pubmed/19179559
http://dx.doi.org/10.1093/jxb/ern338
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author Prudent, Marion
Causse, Mathilde
Génard, Michel
Tripodi, Pasquale
Grandillo, Silvana
Bertin, Nadia
author_facet Prudent, Marion
Causse, Mathilde
Génard, Michel
Tripodi, Pasquale
Grandillo, Silvana
Bertin, Nadia
author_sort Prudent, Marion
collection PubMed
description Throughout tomato domestication, a large increase in fruit size was associated with a loss of dry matter and sugar contents. This study aims to dissect the contributions of genetic variation and the physiological processes underlying the relationships between fruit growth and the accumulation of dry matter and sugars. Fruit quality traits and physiological parameters were measured on 20 introgression lines derived from the introgression of Solanum chmielewskii into S. lycopersicum, under high (HL, unpruned trusses) and low (LL, trusses pruned to one fruit) fruit load conditions. Inter- and intra-genotypic correlations among traits were estimated and quantitative trait loci (QTL) for size, composition, and physiological traits were mapped. LL increased almost all traits, but the response of sugar content was genotype-dependent, involving either dilution effects or differences in carbon allocation to sugars. Genotype×fruit load interactions were significant for most traits and only 30% of the QTL were stable under both fruit loads. Many QTL for fresh weight and cell or seed numbers co-localized. Eleven clusters of QTL for fresh weight and dry matter or sugar content were detected, eight with opposite allele effects and three with negative effects. Two genotypic antagonistic relationships, between fresh weight and dry matter content and between cell number and cell size, were significant only under HL; the second could be interpreted as a competition for carbohydrates among cells. The role of cuticular conductance, fruit transpiration or cracking in the relationship between fruit fresh weight and composition was also emphasized at the genetic and physiological levels.
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spelling pubmed-26520582009-04-02 Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection Prudent, Marion Causse, Mathilde Génard, Michel Tripodi, Pasquale Grandillo, Silvana Bertin, Nadia J Exp Bot Research Papers Throughout tomato domestication, a large increase in fruit size was associated with a loss of dry matter and sugar contents. This study aims to dissect the contributions of genetic variation and the physiological processes underlying the relationships between fruit growth and the accumulation of dry matter and sugars. Fruit quality traits and physiological parameters were measured on 20 introgression lines derived from the introgression of Solanum chmielewskii into S. lycopersicum, under high (HL, unpruned trusses) and low (LL, trusses pruned to one fruit) fruit load conditions. Inter- and intra-genotypic correlations among traits were estimated and quantitative trait loci (QTL) for size, composition, and physiological traits were mapped. LL increased almost all traits, but the response of sugar content was genotype-dependent, involving either dilution effects or differences in carbon allocation to sugars. Genotype×fruit load interactions were significant for most traits and only 30% of the QTL were stable under both fruit loads. Many QTL for fresh weight and cell or seed numbers co-localized. Eleven clusters of QTL for fresh weight and dry matter or sugar content were detected, eight with opposite allele effects and three with negative effects. Two genotypic antagonistic relationships, between fresh weight and dry matter content and between cell number and cell size, were significant only under HL; the second could be interpreted as a competition for carbohydrates among cells. The role of cuticular conductance, fruit transpiration or cracking in the relationship between fruit fresh weight and composition was also emphasized at the genetic and physiological levels. Oxford University Press 2009-03 2009-01-29 /pmc/articles/PMC2652058/ /pubmed/19179559 http://dx.doi.org/10.1093/jxb/ern338 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Prudent, Marion
Causse, Mathilde
Génard, Michel
Tripodi, Pasquale
Grandillo, Silvana
Bertin, Nadia
Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title_full Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title_fullStr Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title_full_unstemmed Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title_short Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection
title_sort genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on qtl detection
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652058/
https://www.ncbi.nlm.nih.gov/pubmed/19179559
http://dx.doi.org/10.1093/jxb/ern338
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