Vitrification of isolated mice blastomeres using a closed loading device

Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A lot of developing technologies may benefit by the efficient cryopreservation of blastomeres for futur...

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Detalles Bibliográficos
Autores principales: Kader, Amr, Agarwal, Ashok, Sharma, Rakesh, Falcone, Tommaso
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652462/
https://www.ncbi.nlm.nih.gov/pubmed/19228397
http://dx.doi.org/10.1186/1477-7827-7-17
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author Kader, Amr
Agarwal, Ashok
Sharma, Rakesh
Falcone, Tommaso
author_facet Kader, Amr
Agarwal, Ashok
Sharma, Rakesh
Falcone, Tommaso
author_sort Kader, Amr
collection PubMed
description Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A lot of developing technologies may benefit by the efficient cryopreservation of blastomeres for future potential use, especially for stem cell culture and differentiation control. We are hereby reporting for the first time the feasibility of preserving individual isolated blastomeres in microvolumes in a closed vitrification system. Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival.
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spelling pubmed-26524622009-03-07 Vitrification of isolated mice blastomeres using a closed loading device Kader, Amr Agarwal, Ashok Sharma, Rakesh Falcone, Tommaso Reprod Biol Endocrinol Methodology Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A lot of developing technologies may benefit by the efficient cryopreservation of blastomeres for future potential use, especially for stem cell culture and differentiation control. We are hereby reporting for the first time the feasibility of preserving individual isolated blastomeres in microvolumes in a closed vitrification system. Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival. BioMed Central 2009-02-19 /pmc/articles/PMC2652462/ /pubmed/19228397 http://dx.doi.org/10.1186/1477-7827-7-17 Text en Copyright © 2009 Kader et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Kader, Amr
Agarwal, Ashok
Sharma, Rakesh
Falcone, Tommaso
Vitrification of isolated mice blastomeres using a closed loading device
title Vitrification of isolated mice blastomeres using a closed loading device
title_full Vitrification of isolated mice blastomeres using a closed loading device
title_fullStr Vitrification of isolated mice blastomeres using a closed loading device
title_full_unstemmed Vitrification of isolated mice blastomeres using a closed loading device
title_short Vitrification of isolated mice blastomeres using a closed loading device
title_sort vitrification of isolated mice blastomeres using a closed loading device
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652462/
https://www.ncbi.nlm.nih.gov/pubmed/19228397
http://dx.doi.org/10.1186/1477-7827-7-17
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AT sharmarakesh vitrificationofisolatedmiceblastomeresusingaclosedloadingdevice
AT falconetommaso vitrificationofisolatedmiceblastomeresusingaclosedloadingdevice

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