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Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds
Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled a...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652471/ https://www.ncbi.nlm.nih.gov/pubmed/19226456 http://dx.doi.org/10.1186/1746-1448-5-2 |
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author | Ben-Dov, Eitan Kushmaro, Ariel Brenner, Asher |
author_facet | Ben-Dov, Eitan Kushmaro, Ariel Brenner, Asher |
author_sort | Ben-Dov, Eitan |
collection | PubMed |
description | Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled and the presence of SRB was determined by quantitative real-time PCR (qPCR) with a set of primers designed to amplify the dissimilatory sulfite reductase (dsrA) gene. SRB displayed higher abundance during the summer (10(6)–10(8 )targets ml(-1)) and lower abundance from the autumn-spring (10(3)–10(5 )targets ml(-1)). However, addition of concentrated dissolved organic matter into the evaporation ponds during winter immediately resulted in a proliferation of SRB, despite the lower wastewater temperature (12–14°C). These results indicate that the qPCR approach can be used for rapid measurement of SRB to provide valuable information about the abundance of SRB in harsh environments, such as highly saline industrial wastewaters. Low level of H(2)S has been maintained over five years, which indicates a possible inhibition of SRB activity, following artificial salination (≈16% w/v of NaCl) of wastewater evaporation ponds, despite SRB reproduction being detected by qPCR. |
format | Text |
id | pubmed-2652471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26524712009-03-07 Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds Ben-Dov, Eitan Kushmaro, Ariel Brenner, Asher Saline Syst Short Report Abundance and seasonal dynamics of sulfate-reducing bacteria (SRB), in general, and of extreme halophilic SRB (belonging to Desulfocella halophila) in particular, were examined in highly saline industrial wastewater evaporation ponds over a forty one month period. Industrial wastewater was sampled and the presence of SRB was determined by quantitative real-time PCR (qPCR) with a set of primers designed to amplify the dissimilatory sulfite reductase (dsrA) gene. SRB displayed higher abundance during the summer (10(6)–10(8 )targets ml(-1)) and lower abundance from the autumn-spring (10(3)–10(5 )targets ml(-1)). However, addition of concentrated dissolved organic matter into the evaporation ponds during winter immediately resulted in a proliferation of SRB, despite the lower wastewater temperature (12–14°C). These results indicate that the qPCR approach can be used for rapid measurement of SRB to provide valuable information about the abundance of SRB in harsh environments, such as highly saline industrial wastewaters. Low level of H(2)S has been maintained over five years, which indicates a possible inhibition of SRB activity, following artificial salination (≈16% w/v of NaCl) of wastewater evaporation ponds, despite SRB reproduction being detected by qPCR. BioMed Central 2009-02-18 /pmc/articles/PMC2652471/ /pubmed/19226456 http://dx.doi.org/10.1186/1746-1448-5-2 Text en Copyright © 2009 Ben-Dov et al; licensee BioMed Central Ltd. https://creativecommons.org/licenses/by/2.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0 (https://creativecommons.org/licenses/by/2.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Ben-Dov, Eitan Kushmaro, Ariel Brenner, Asher Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title | Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title_full | Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title_fullStr | Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title_full_unstemmed | Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title_short | Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
title_sort | long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652471/ https://www.ncbi.nlm.nih.gov/pubmed/19226456 http://dx.doi.org/10.1186/1746-1448-5-2 |
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