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An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies

BACKGROUND: The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selec...

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Autores principales: Frentiu, Francesca D, Adamski, Marcin, McGraw, Elizabeth A, Blows, Mark W, Chenoweth, Stephen F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2653539/
https://www.ncbi.nlm.nih.gov/pubmed/19159479
http://dx.doi.org/10.1186/1471-2164-10-40
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author Frentiu, Francesca D
Adamski, Marcin
McGraw, Elizabeth A
Blows, Mark W
Chenoweth, Stephen F
author_facet Frentiu, Francesca D
Adamski, Marcin
McGraw, Elizabeth A
Blows, Mark W
Chenoweth, Stephen F
author_sort Frentiu, Francesca D
collection PubMed
description BACKGROUND: The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST) collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. RESULTS: A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs), with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at . CONCLUSION: We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST collection will provide much needed genomic resources for this model species and facilitate comparative evolutionary studies within the montium subgroup of the D. melanogaster lineage.
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spelling pubmed-26535392009-03-10 An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies Frentiu, Francesca D Adamski, Marcin McGraw, Elizabeth A Blows, Mark W Chenoweth, Stephen F BMC Genomics Research Article BACKGROUND: The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST) collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. RESULTS: A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs), with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at . CONCLUSION: We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST collection will provide much needed genomic resources for this model species and facilitate comparative evolutionary studies within the montium subgroup of the D. melanogaster lineage. BioMed Central 2009-01-21 /pmc/articles/PMC2653539/ /pubmed/19159479 http://dx.doi.org/10.1186/1471-2164-10-40 Text en Copyright © 2009 Frentiu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Frentiu, Francesca D
Adamski, Marcin
McGraw, Elizabeth A
Blows, Mark W
Chenoweth, Stephen F
An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title_full An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title_fullStr An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title_full_unstemmed An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title_short An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies
title_sort expressed sequence tag (est) library for drosophila serrata, a model system for sexual selection and climatic adaptation studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2653539/
https://www.ncbi.nlm.nih.gov/pubmed/19159479
http://dx.doi.org/10.1186/1471-2164-10-40
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