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Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ

Telomeres protect chromosome ends from being viewed as double-strand breaks and from eliciting a DNA damage response. Deprotection of chromosome ends occurs when telomeres become critically short because of replicative attrition or inhibition of TRF2. In this study, we report a novel form of deprote...

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Detalles Bibliográficos
Autores principales: Hsiao, Susan J., Smith, Susan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654126/
https://www.ncbi.nlm.nih.gov/pubmed/19221198
http://dx.doi.org/10.1083/jcb.200810132
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author Hsiao, Susan J.
Smith, Susan
author_facet Hsiao, Susan J.
Smith, Susan
author_sort Hsiao, Susan J.
collection PubMed
description Telomeres protect chromosome ends from being viewed as double-strand breaks and from eliciting a DNA damage response. Deprotection of chromosome ends occurs when telomeres become critically short because of replicative attrition or inhibition of TRF2. In this study, we report a novel form of deprotection that occurs exclusively after DNA replication in S/G2 phase of the cell cycle. In cells deficient in the telomeric poly(adenosine diphosphate ribose) polymerase tankyrase 1, sister telomere resolution is blocked. Unexpectedly, cohered sister telomeres become deprotected and are inappropriately fused. In contrast to telomeres rendered dysfunctional by TRF2, which engage in chromatid fusions predominantly between chromatids from different chromosomes (Bailey, S.M., M.N. Cornforth, A. Kurimasa, D.J. Chen, and E.H. Goodwin. 2001. Science. 293:2462–2465; Smogorzewska, A., J. Karlseder, H. Holtgreve-Grez, A. Jauch, and T. de Lange. 2002. Curr. Biol. 12:1635–1644), telomeres rendered dysfunctional by tankyrase 1 engage in chromatid fusions almost exclusively between sister chromatids. We show that cohered sister telomeres are fused by DNA ligase IV–mediated nonhomologous end joining. These results demonstrate that the timely removal of sister telomere cohesion is essential for the formation of a protective structure at chromosome ends after DNA replication in S/G2 phase of the cell cycle.
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spelling pubmed-26541262009-08-23 Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ Hsiao, Susan J. Smith, Susan J Cell Biol Research Articles Telomeres protect chromosome ends from being viewed as double-strand breaks and from eliciting a DNA damage response. Deprotection of chromosome ends occurs when telomeres become critically short because of replicative attrition or inhibition of TRF2. In this study, we report a novel form of deprotection that occurs exclusively after DNA replication in S/G2 phase of the cell cycle. In cells deficient in the telomeric poly(adenosine diphosphate ribose) polymerase tankyrase 1, sister telomere resolution is blocked. Unexpectedly, cohered sister telomeres become deprotected and are inappropriately fused. In contrast to telomeres rendered dysfunctional by TRF2, which engage in chromatid fusions predominantly between chromatids from different chromosomes (Bailey, S.M., M.N. Cornforth, A. Kurimasa, D.J. Chen, and E.H. Goodwin. 2001. Science. 293:2462–2465; Smogorzewska, A., J. Karlseder, H. Holtgreve-Grez, A. Jauch, and T. de Lange. 2002. Curr. Biol. 12:1635–1644), telomeres rendered dysfunctional by tankyrase 1 engage in chromatid fusions almost exclusively between sister chromatids. We show that cohered sister telomeres are fused by DNA ligase IV–mediated nonhomologous end joining. These results demonstrate that the timely removal of sister telomere cohesion is essential for the formation of a protective structure at chromosome ends after DNA replication in S/G2 phase of the cell cycle. The Rockefeller University Press 2009-02-23 /pmc/articles/PMC2654126/ /pubmed/19221198 http://dx.doi.org/10.1083/jcb.200810132 Text en © 2009 Hsiao and Smith This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Hsiao, Susan J.
Smith, Susan
Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title_full Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title_fullStr Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title_full_unstemmed Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title_short Sister telomeres rendered dysfunctional by persistent cohesion are fused by NHEJ
title_sort sister telomeres rendered dysfunctional by persistent cohesion are fused by nhej
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654126/
https://www.ncbi.nlm.nih.gov/pubmed/19221198
http://dx.doi.org/10.1083/jcb.200810132
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