Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors

Drug-inducible lentiviruses encoding Oct4, Sox2, Klf4 and c-Myc were used to derive “primary” iPS cells and segregated through germline transmission generating mice and fibroblasts carrying subsets of the reprogramming factors. Drug treatment of the cells resulted in “secondary” iPS cell derivation...

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Autores principales: Markoulaki, Styliani, Hanna, Jacob, Beard, Caroline, Carey, Bryce W., Cheng, Albert W., Lengner, Christopher J., Dausman, Jessica A., Fu, Dongdong, Gao, Qing, Wu, Su, Cassady, John P., Jaenisch, Rudolf
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654270/
https://www.ncbi.nlm.nih.gov/pubmed/19151700
http://dx.doi.org/10.1038/nbt.1520
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author Markoulaki, Styliani
Hanna, Jacob
Beard, Caroline
Carey, Bryce W.
Cheng, Albert W.
Lengner, Christopher J.
Dausman, Jessica A.
Fu, Dongdong
Gao, Qing
Wu, Su
Cassady, John P.
Jaenisch, Rudolf
author_facet Markoulaki, Styliani
Hanna, Jacob
Beard, Caroline
Carey, Bryce W.
Cheng, Albert W.
Lengner, Christopher J.
Dausman, Jessica A.
Fu, Dongdong
Gao, Qing
Wu, Su
Cassady, John P.
Jaenisch, Rudolf
author_sort Markoulaki, Styliani
collection PubMed
description Drug-inducible lentiviruses encoding Oct4, Sox2, Klf4 and c-Myc were used to derive “primary” iPS cells and segregated through germline transmission generating mice and fibroblasts carrying subsets of the reprogramming factors. Drug treatment of the cells resulted in “secondary” iPS cell derivation only when the missing factor was introduced. This creates a defined platform for studying reprogramming mechanisms and allows screening of genetically homogenous cells for compounds that replace any transcription factor required for iPS cell derivation.
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spelling pubmed-26542702009-08-01 Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors Markoulaki, Styliani Hanna, Jacob Beard, Caroline Carey, Bryce W. Cheng, Albert W. Lengner, Christopher J. Dausman, Jessica A. Fu, Dongdong Gao, Qing Wu, Su Cassady, John P. Jaenisch, Rudolf Nat Biotechnol Article Drug-inducible lentiviruses encoding Oct4, Sox2, Klf4 and c-Myc were used to derive “primary” iPS cells and segregated through germline transmission generating mice and fibroblasts carrying subsets of the reprogramming factors. Drug treatment of the cells resulted in “secondary” iPS cell derivation only when the missing factor was introduced. This creates a defined platform for studying reprogramming mechanisms and allows screening of genetically homogenous cells for compounds that replace any transcription factor required for iPS cell derivation. 2009-01-18 2009-02 /pmc/articles/PMC2654270/ /pubmed/19151700 http://dx.doi.org/10.1038/nbt.1520 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Markoulaki, Styliani
Hanna, Jacob
Beard, Caroline
Carey, Bryce W.
Cheng, Albert W.
Lengner, Christopher J.
Dausman, Jessica A.
Fu, Dongdong
Gao, Qing
Wu, Su
Cassady, John P.
Jaenisch, Rudolf
Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title_full Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title_fullStr Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title_full_unstemmed Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title_short Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors
title_sort transgenic mice with defined combinations of drug inducible reprogramming factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654270/
https://www.ncbi.nlm.nih.gov/pubmed/19151700
http://dx.doi.org/10.1038/nbt.1520
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