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Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy

BACKGROUND: Inflammation contributes to cardiovascular complications in type 2 diabetes, which are often characterized by microvascular alterations. We investigated whether myeloid-related protein 8/14 complex (MRP8/14) secreted by transmigrating monocytes and granulocytes may represent a biomarker...

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Autores principales: Burkhardt, Klaus, Schwarz, Sonja, Pan, Chengrui, Stelter, Felix, Kotliar, Konstantin, Von Eynatten, Maxilian, Sollinger, Daniel, Lanzl, Ines, Heemann, Uwe, Baumann, Marcus
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654885/
https://www.ncbi.nlm.nih.gov/pubmed/19232095
http://dx.doi.org/10.1186/1475-2840-8-10
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author Burkhardt, Klaus
Schwarz, Sonja
Pan, Chengrui
Stelter, Felix
Kotliar, Konstantin
Von Eynatten, Maxilian
Sollinger, Daniel
Lanzl, Ines
Heemann, Uwe
Baumann, Marcus
author_facet Burkhardt, Klaus
Schwarz, Sonja
Pan, Chengrui
Stelter, Felix
Kotliar, Konstantin
Von Eynatten, Maxilian
Sollinger, Daniel
Lanzl, Ines
Heemann, Uwe
Baumann, Marcus
author_sort Burkhardt, Klaus
collection PubMed
description BACKGROUND: Inflammation contributes to cardiovascular complications in type 2 diabetes, which are often characterized by microvascular alterations. We investigated whether myeloid-related protein 8/14 complex (MRP8/14) secreted by transmigrating monocytes and granulocytes may represent a biomarker for microvascular alterations in patients with type 2 diabetes and nephropathy. METHODS: MRP8/14 was measured in 43 patients with type 2 diabetes and nephropathy. Additionally, the inflammatory markers Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were quantified. To detect microvascular alterations proteinuria and retinal vessel caliber were used as classical and novel marker, respectively. Proteinuria was quantified by protein-creatinine ratio (PCR); retinal vessel caliber was quantified after retina photography on digitalized retina pictures. RESULTS: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48). Type 2 diabetic patients with MRP8/14 values above the median of 5.8 μg/ml demonstrated higher proteinuria and larger retinal artery caliber than patients with MRP8/14 values below the median (logPCR: -0.51 ± 0.52 versus -0.96 ± 0.46, P < 0.01; retinal artery lumen (μm): 178.3 ± 14.1 versus 162.7 ± 14.9 P < 0.01). Both groups did not differ with regard to metabolic factors and blood pressure. MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (β = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-α (r = 0.36, P < 0.05). CONCLUSION: MRP8/14 – a marker for transendothelial migration – describes not only the state of inflammation in diabetic nephropathy, but additionally the degree of microvascular alterations in the glomerular and retinal bed. Therefore, MRP8/14 may be a potentially selective novel biomarker for microcirculatory defects in diabetic nephropathy.
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spelling pubmed-26548852009-03-13 Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy Burkhardt, Klaus Schwarz, Sonja Pan, Chengrui Stelter, Felix Kotliar, Konstantin Von Eynatten, Maxilian Sollinger, Daniel Lanzl, Ines Heemann, Uwe Baumann, Marcus Cardiovasc Diabetol Original Investigation BACKGROUND: Inflammation contributes to cardiovascular complications in type 2 diabetes, which are often characterized by microvascular alterations. We investigated whether myeloid-related protein 8/14 complex (MRP8/14) secreted by transmigrating monocytes and granulocytes may represent a biomarker for microvascular alterations in patients with type 2 diabetes and nephropathy. METHODS: MRP8/14 was measured in 43 patients with type 2 diabetes and nephropathy. Additionally, the inflammatory markers Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were quantified. To detect microvascular alterations proteinuria and retinal vessel caliber were used as classical and novel marker, respectively. Proteinuria was quantified by protein-creatinine ratio (PCR); retinal vessel caliber was quantified after retina photography on digitalized retina pictures. RESULTS: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48). Type 2 diabetic patients with MRP8/14 values above the median of 5.8 μg/ml demonstrated higher proteinuria and larger retinal artery caliber than patients with MRP8/14 values below the median (logPCR: -0.51 ± 0.52 versus -0.96 ± 0.46, P < 0.01; retinal artery lumen (μm): 178.3 ± 14.1 versus 162.7 ± 14.9 P < 0.01). Both groups did not differ with regard to metabolic factors and blood pressure. MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (β = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-α (r = 0.36, P < 0.05). CONCLUSION: MRP8/14 – a marker for transendothelial migration – describes not only the state of inflammation in diabetic nephropathy, but additionally the degree of microvascular alterations in the glomerular and retinal bed. Therefore, MRP8/14 may be a potentially selective novel biomarker for microcirculatory defects in diabetic nephropathy. BioMed Central 2009-02-20 /pmc/articles/PMC2654885/ /pubmed/19232095 http://dx.doi.org/10.1186/1475-2840-8-10 Text en Copyright © 2009 Burkhardt et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Investigation
Burkhardt, Klaus
Schwarz, Sonja
Pan, Chengrui
Stelter, Felix
Kotliar, Konstantin
Von Eynatten, Maxilian
Sollinger, Daniel
Lanzl, Ines
Heemann, Uwe
Baumann, Marcus
Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title_full Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title_fullStr Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title_full_unstemmed Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title_short Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
title_sort myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654885/
https://www.ncbi.nlm.nih.gov/pubmed/19232095
http://dx.doi.org/10.1186/1475-2840-8-10
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