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Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish

The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular a...

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Detalles Bibliográficos
Autores principales: Gomez, Gustavo A., Veldman, Matthew B., Zhao, Yan, Burgess, Shawn, Lin, Shuo
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654924/
https://www.ncbi.nlm.nih.gov/pubmed/19308258
http://dx.doi.org/10.1371/journal.pone.0004994
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author Gomez, Gustavo A.
Veldman, Matthew B.
Zhao, Yan
Burgess, Shawn
Lin, Shuo
author_facet Gomez, Gustavo A.
Veldman, Matthew B.
Zhao, Yan
Burgess, Shawn
Lin, Shuo
author_sort Gomez, Gustavo A.
collection PubMed
description The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular and hematopoietic development. To identify genes downstream of etsrp, gain-of-function experiments were performed for etsrp in zebrafish embryos followed by transcription profile analysis by microarray. Subsequent in vivo expression studies resulted in the identification of fourteen genes with blood and/or vascular expression, six of these being completely novel. Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos. Additional functional analysis of two newly discovered genes, hapln1b and sh3gl3, demonstrates their importance in embryonic vascular development. The results described here identify a group of genes downstream of etsrp likely to be critical for vascular and/or myeloid development.
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spelling pubmed-26549242009-03-24 Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish Gomez, Gustavo A. Veldman, Matthew B. Zhao, Yan Burgess, Shawn Lin, Shuo PLoS One Research Article The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular and hematopoietic development. To identify genes downstream of etsrp, gain-of-function experiments were performed for etsrp in zebrafish embryos followed by transcription profile analysis by microarray. Subsequent in vivo expression studies resulted in the identification of fourteen genes with blood and/or vascular expression, six of these being completely novel. Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos. Additional functional analysis of two newly discovered genes, hapln1b and sh3gl3, demonstrates their importance in embryonic vascular development. The results described here identify a group of genes downstream of etsrp likely to be critical for vascular and/or myeloid development. Public Library of Science 2009-03-24 /pmc/articles/PMC2654924/ /pubmed/19308258 http://dx.doi.org/10.1371/journal.pone.0004994 Text en Lin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gomez, Gustavo A.
Veldman, Matthew B.
Zhao, Yan
Burgess, Shawn
Lin, Shuo
Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title_full Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title_fullStr Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title_full_unstemmed Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title_short Discovery and Characterization of Novel Vascular and Hematopoietic Genes Downstream of Etsrp in Zebrafish
title_sort discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654924/
https://www.ncbi.nlm.nih.gov/pubmed/19308258
http://dx.doi.org/10.1371/journal.pone.0004994
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