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Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectors
BACKGROUND: Overexpression of proteins in Escherichia coli is considered routine today, at least when the protein is soluble and not otherwise toxic for the host. We report here that the massive overproduction of even such "benign" proteins can cause surprisingly efficient promoter deletio...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655282/ https://www.ncbi.nlm.nih.gov/pubmed/19171063 http://dx.doi.org/10.1186/1475-2859-8-8 |