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Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation
Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. Cigarette smoke has been considered a major player in the pathogenesis of COPD. The inflamed airways of COPD patients contain several inflammatory cells including neutrophils, macrophages,T lymphocytes, and...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655711/ https://www.ncbi.nlm.nih.gov/pubmed/19293939 http://dx.doi.org/10.1371/journal.pone.0004946 |
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author | Mortaz, Esmaeil Kraneveld, Aletta D. Smit, Joost J. Kool, Mirjam Lambrecht, Bart N. Kunkel, Steven L. Lukacs, Nicholas W. Nijkamp, Frans P. Folkerts, Gert |
author_facet | Mortaz, Esmaeil Kraneveld, Aletta D. Smit, Joost J. Kool, Mirjam Lambrecht, Bart N. Kunkel, Steven L. Lukacs, Nicholas W. Nijkamp, Frans P. Folkerts, Gert |
author_sort | Mortaz, Esmaeil |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. Cigarette smoke has been considered a major player in the pathogenesis of COPD. The inflamed airways of COPD patients contain several inflammatory cells including neutrophils, macrophages,T lymphocytes, and dendritic cells (DCs). The relative contributions of these various inflammatory cells to airway injury and remodeling are not well documented. In particular, the potential role of DCs as mediators of inflammation in the smoker's airways and COPD patients is poorly understood. In the current study we analyzed the effects of cigarette smoke extract on mouse bone marrow derived DC and the production of chemokines and cytokines were studied. In addition, we assessed CSE-induced changes in cDC function in the mixed lymphocyte reaction (MLR) examining CD4+ and CD8+ T cell proliferation. Cigarette smoke extract induces the release of the chemokines CCL3 and CXCL2 (but not cytokines), via the generation of reactive oxygen species (ROS). In a mixed-leukocyte reaction assay, cigarette smoke-primed DCs potentiate CD8(+)T cell proliferation via CCL3. In contrast, proliferation of CD4(+)T cells is suppressed via an unknown mechanism. The cigarette smoke-induced release of CCL3 and CXCL2 by DCs may contribute to the influx of CD8(+)T cells and neutrophils into the airways, respectively. |
format | Text |
id | pubmed-2655711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26557112009-03-18 Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation Mortaz, Esmaeil Kraneveld, Aletta D. Smit, Joost J. Kool, Mirjam Lambrecht, Bart N. Kunkel, Steven L. Lukacs, Nicholas W. Nijkamp, Frans P. Folkerts, Gert PLoS One Research Article Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. Cigarette smoke has been considered a major player in the pathogenesis of COPD. The inflamed airways of COPD patients contain several inflammatory cells including neutrophils, macrophages,T lymphocytes, and dendritic cells (DCs). The relative contributions of these various inflammatory cells to airway injury and remodeling are not well documented. In particular, the potential role of DCs as mediators of inflammation in the smoker's airways and COPD patients is poorly understood. In the current study we analyzed the effects of cigarette smoke extract on mouse bone marrow derived DC and the production of chemokines and cytokines were studied. In addition, we assessed CSE-induced changes in cDC function in the mixed lymphocyte reaction (MLR) examining CD4+ and CD8+ T cell proliferation. Cigarette smoke extract induces the release of the chemokines CCL3 and CXCL2 (but not cytokines), via the generation of reactive oxygen species (ROS). In a mixed-leukocyte reaction assay, cigarette smoke-primed DCs potentiate CD8(+)T cell proliferation via CCL3. In contrast, proliferation of CD4(+)T cells is suppressed via an unknown mechanism. The cigarette smoke-induced release of CCL3 and CXCL2 by DCs may contribute to the influx of CD8(+)T cells and neutrophils into the airways, respectively. Public Library of Science 2009-03-18 /pmc/articles/PMC2655711/ /pubmed/19293939 http://dx.doi.org/10.1371/journal.pone.0004946 Text en Mortaz et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mortaz, Esmaeil Kraneveld, Aletta D. Smit, Joost J. Kool, Mirjam Lambrecht, Bart N. Kunkel, Steven L. Lukacs, Nicholas W. Nijkamp, Frans P. Folkerts, Gert Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title | Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title_full | Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title_fullStr | Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title_full_unstemmed | Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title_short | Effect of Cigarette Smoke Extract on Dendritic Cells and Their Impact on T-Cell Proliferation |
title_sort | effect of cigarette smoke extract on dendritic cells and their impact on t-cell proliferation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655711/ https://www.ncbi.nlm.nih.gov/pubmed/19293939 http://dx.doi.org/10.1371/journal.pone.0004946 |
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