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Surface plasmon resonance imaging of cells and surface-associated fibronectin
BACKGROUND: A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for examining cell-matrix interactions. A label-free i...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2656462/ https://www.ncbi.nlm.nih.gov/pubmed/19245706 http://dx.doi.org/10.1186/1471-2121-10-16 |
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author | Peterson, Alexander W Halter, Michael Tona, Alessandro Bhadriraju, Kiran Plant, Anne L |
author_facet | Peterson, Alexander W Halter, Michael Tona, Alessandro Bhadriraju, Kiran Plant, Anne L |
author_sort | Peterson, Alexander W |
collection | PubMed |
description | BACKGROUND: A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for examining cell-matrix interactions. A label-free imaging method would provide an alternative that would eliminate the requirement of transfected cells and modified biological molecules, and if collected nondestructively, would allow long term observation and analysis of live cells. RESULTS: Using surface plasmon resonance imaging (SPRI), the deposition of protein by vascular smooth muscle cells (vSMC) cultured on fibronectin was quantified as a function of cell density and distance from the cell periphery. We observed that as much as 120 ng/cm(2 )of protein was deposited by cells in 24 h. CONCLUSION: SPRI is a real-time, low-light-level, label-free imaging technique that allows the simultaneous observation and quantification of protein layers and cellular features. This technique is compatible with live cells such that it is possible to monitor cellular modifications to the extracellular matrix in real-time. |
format | Text |
id | pubmed-2656462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26564622009-03-17 Surface plasmon resonance imaging of cells and surface-associated fibronectin Peterson, Alexander W Halter, Michael Tona, Alessandro Bhadriraju, Kiran Plant, Anne L BMC Cell Biol Methodology Article BACKGROUND: A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for examining cell-matrix interactions. A label-free imaging method would provide an alternative that would eliminate the requirement of transfected cells and modified biological molecules, and if collected nondestructively, would allow long term observation and analysis of live cells. RESULTS: Using surface plasmon resonance imaging (SPRI), the deposition of protein by vascular smooth muscle cells (vSMC) cultured on fibronectin was quantified as a function of cell density and distance from the cell periphery. We observed that as much as 120 ng/cm(2 )of protein was deposited by cells in 24 h. CONCLUSION: SPRI is a real-time, low-light-level, label-free imaging technique that allows the simultaneous observation and quantification of protein layers and cellular features. This technique is compatible with live cells such that it is possible to monitor cellular modifications to the extracellular matrix in real-time. BioMed Central 2009-02-26 /pmc/articles/PMC2656462/ /pubmed/19245706 http://dx.doi.org/10.1186/1471-2121-10-16 Text en Copyright © 2009 Peterson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Peterson, Alexander W Halter, Michael Tona, Alessandro Bhadriraju, Kiran Plant, Anne L Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title | Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title_full | Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title_fullStr | Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title_full_unstemmed | Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title_short | Surface plasmon resonance imaging of cells and surface-associated fibronectin |
title_sort | surface plasmon resonance imaging of cells and surface-associated fibronectin |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2656462/ https://www.ncbi.nlm.nih.gov/pubmed/19245706 http://dx.doi.org/10.1186/1471-2121-10-16 |
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