Cargando…
Functional characterization of E- and P-cadherin in invasive breast cancer cells
BACKGROUND: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. METHODS: To compare the functional role of E-cadherin a...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2656544/ https://www.ncbi.nlm.nih.gov/pubmed/19257890 http://dx.doi.org/10.1186/1471-2407-9-74 |
_version_ | 1782165523719520256 |
---|---|
author | Sarrió, David Palacios, José Hergueta-Redondo, Marta Gómez-López, Gonzalo Cano, Amparo Moreno-Bueno, Gema |
author_facet | Sarrió, David Palacios, José Hergueta-Redondo, Marta Gómez-López, Gonzalo Cano, Amparo Moreno-Bueno, Gema |
author_sort | Sarrió, David |
collection | PubMed |
description | BACKGROUND: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. METHODS: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation. RESULTS: Expression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type. CONCLUSION: E- and P-cadherin have similar functional consequences on the phenotype and invasive behavior of MDA-MB-231 cells. Moreover, we demonstrate for the first time that these cadherins can induce both common and specific gene expression programs on invasive breast cancer cells. Importantly, these identified genes are potential targets for future studies on the functional consequences of altered cadherin expression in human breast cancer. |
format | Text |
id | pubmed-2656544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26565442009-03-17 Functional characterization of E- and P-cadherin in invasive breast cancer cells Sarrió, David Palacios, José Hergueta-Redondo, Marta Gómez-López, Gonzalo Cano, Amparo Moreno-Bueno, Gema BMC Cancer Research Article BACKGROUND: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. METHODS: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation. RESULTS: Expression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type. CONCLUSION: E- and P-cadherin have similar functional consequences on the phenotype and invasive behavior of MDA-MB-231 cells. Moreover, we demonstrate for the first time that these cadherins can induce both common and specific gene expression programs on invasive breast cancer cells. Importantly, these identified genes are potential targets for future studies on the functional consequences of altered cadherin expression in human breast cancer. BioMed Central 2009-03-03 /pmc/articles/PMC2656544/ /pubmed/19257890 http://dx.doi.org/10.1186/1471-2407-9-74 Text en Copyright ©2009 Sarrió et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sarrió, David Palacios, José Hergueta-Redondo, Marta Gómez-López, Gonzalo Cano, Amparo Moreno-Bueno, Gema Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title | Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title_full | Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title_fullStr | Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title_full_unstemmed | Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title_short | Functional characterization of E- and P-cadherin in invasive breast cancer cells |
title_sort | functional characterization of e- and p-cadherin in invasive breast cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2656544/ https://www.ncbi.nlm.nih.gov/pubmed/19257890 http://dx.doi.org/10.1186/1471-2407-9-74 |
work_keys_str_mv | AT sarriodavid functionalcharacterizationofeandpcadherinininvasivebreastcancercells AT palaciosjose functionalcharacterizationofeandpcadherinininvasivebreastcancercells AT herguetaredondomarta functionalcharacterizationofeandpcadherinininvasivebreastcancercells AT gomezlopezgonzalo functionalcharacterizationofeandpcadherinininvasivebreastcancercells AT canoamparo functionalcharacterizationofeandpcadherinininvasivebreastcancercells AT morenobuenogema functionalcharacterizationofeandpcadherinininvasivebreastcancercells |