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Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats

BACKGROUND: A major biomarker for liver fibrosis is transglutaminase which catalyzes cross-linking of epsilon-amines and alpha-glutamyl residues among amino acids leading to fibrosis. Fructus Piperis Longi is a common herb used in Chinese medicine. The present study evaluates the role of the ethanol...

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Autores principales: Mansour, Somaya Zakaria, El-Kabany, Hanan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657146/
https://www.ncbi.nlm.nih.gov/pubmed/19183455
http://dx.doi.org/10.1186/1749-8546-4-2
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author Mansour, Somaya Zakaria
El-Kabany, Hanan
author_facet Mansour, Somaya Zakaria
El-Kabany, Hanan
author_sort Mansour, Somaya Zakaria
collection PubMed
description BACKGROUND: A major biomarker for liver fibrosis is transglutaminase which catalyzes cross-linking of epsilon-amines and alpha-glutamyl residues among amino acids leading to fibrosis. Fructus Piperis Longi is a common herb used in Chinese medicine. The present study evaluates the role of the ethanol extract of Fructus Piperis Longi in the modulation of liver function in liver fibrosis. METHODS: Plf extract (50 mg/kg) was force-fed to rats every other day 7 days before administration of thioacetamide and/or gamma irradiation. Thioacetamid 200 mg/kg was intraperitoneally administered to rats twice per week for four weeks. Rats were gamma irradiated (2 Gy/week up to a total dose of 8 Gy). Administration of Plf ext was extended during thioacetamid and/or irradiation treatment. Animals were sacrificed. Biochemical parameters in homogenised liver were tested. RESULTS: A significant increase in transglutaminase activity and collagen content was recorded in the liver of thioacetamid-treated and/or irradiated rats. Significant increases in lipid peroxides, lipid hydroperoxides and conjugated dienes associated to significant decreases of reduced glutathione content, superoxide dismutase and catalase activities were also recorded. Administration of Plf ext treatment reduced the severity of liver fibrosis and oxidative damage which was substantiated by amelioration of liver function detected by a decrease in serum aspartate aminotransaminase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase activities and bilirubin (total, direct and indirect) content. CONCLUSION: Treatment of the ethanolic extract of Fructus Piperis Longi ameliorated the increase of the activity of tTG enzyme and enhanced antioxidant activities in fibrotic liver.
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spelling pubmed-26571462009-03-18 Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats Mansour, Somaya Zakaria El-Kabany, Hanan Chin Med Research BACKGROUND: A major biomarker for liver fibrosis is transglutaminase which catalyzes cross-linking of epsilon-amines and alpha-glutamyl residues among amino acids leading to fibrosis. Fructus Piperis Longi is a common herb used in Chinese medicine. The present study evaluates the role of the ethanol extract of Fructus Piperis Longi in the modulation of liver function in liver fibrosis. METHODS: Plf extract (50 mg/kg) was force-fed to rats every other day 7 days before administration of thioacetamide and/or gamma irradiation. Thioacetamid 200 mg/kg was intraperitoneally administered to rats twice per week for four weeks. Rats were gamma irradiated (2 Gy/week up to a total dose of 8 Gy). Administration of Plf ext was extended during thioacetamid and/or irradiation treatment. Animals were sacrificed. Biochemical parameters in homogenised liver were tested. RESULTS: A significant increase in transglutaminase activity and collagen content was recorded in the liver of thioacetamid-treated and/or irradiated rats. Significant increases in lipid peroxides, lipid hydroperoxides and conjugated dienes associated to significant decreases of reduced glutathione content, superoxide dismutase and catalase activities were also recorded. Administration of Plf ext treatment reduced the severity of liver fibrosis and oxidative damage which was substantiated by amelioration of liver function detected by a decrease in serum aspartate aminotransaminase, alanine aminotransferase, alkaline phosphatase, gamma glutamyltransferase activities and bilirubin (total, direct and indirect) content. CONCLUSION: Treatment of the ethanolic extract of Fructus Piperis Longi ameliorated the increase of the activity of tTG enzyme and enhanced antioxidant activities in fibrotic liver. BioMed Central 2009-01-30 /pmc/articles/PMC2657146/ /pubmed/19183455 http://dx.doi.org/10.1186/1749-8546-4-2 Text en Copyright © 2009 Mansour and El-Kabany; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mansour, Somaya Zakaria
El-Kabany, Hanan
Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title_full Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title_fullStr Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title_full_unstemmed Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title_short Effects of Fructus Piperis Longi extract on fibrotic liver of gamma-irradiated rats
title_sort effects of fructus piperis longi extract on fibrotic liver of gamma-irradiated rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657146/
https://www.ncbi.nlm.nih.gov/pubmed/19183455
http://dx.doi.org/10.1186/1749-8546-4-2
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