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Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia

Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus gen...

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Autores principales: Puerta, Alejandro Raul, Ushijima, Koichiro, Koba, Takato, Sassa, Hidenori
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657546/
https://www.ncbi.nlm.nih.gov/pubmed/19282427
http://dx.doi.org/10.1093/jxb/erp005
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author Puerta, Alejandro Raul
Ushijima, Koichiro
Koba, Takato
Sassa, Hidenori
author_facet Puerta, Alejandro Raul
Ushijima, Koichiro
Koba, Takato
Sassa, Hidenori
author_sort Puerta, Alejandro Raul
collection PubMed
description Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus genes, factors located outside the S-locus are also required for expression of GSI. HT-B is one of the pistil non-S-factors identified in Nicotiana and Solanum, and encodes a small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, HT-B was cloned from Petunia and characterized. The structural features and expression pattern of Petunia HT-B were very similar to those of Nicotiana and Solanum. Unlike other solanaceous species, expression of HT-B was also observed in self-compatible Petunia species. RNA interference (RNAi)-mediated suppression of Petunia HT-B resulted in partial breakdown of GSI. Quantitative analysis of the HT-B mRNA accumulation in the transgenics showed that a 100-fold reduction is not sufficient and a >1000-fold reduction is required to achieve partial breakdown of GSI.
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spelling pubmed-26575462009-04-02 Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia Puerta, Alejandro Raul Ushijima, Koichiro Koba, Takato Sassa, Hidenori J Exp Bot Research Papers Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus genes, factors located outside the S-locus are also required for expression of GSI. HT-B is one of the pistil non-S-factors identified in Nicotiana and Solanum, and encodes a small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, HT-B was cloned from Petunia and characterized. The structural features and expression pattern of Petunia HT-B were very similar to those of Nicotiana and Solanum. Unlike other solanaceous species, expression of HT-B was also observed in self-compatible Petunia species. RNA interference (RNAi)-mediated suppression of Petunia HT-B resulted in partial breakdown of GSI. Quantitative analysis of the HT-B mRNA accumulation in the transgenics showed that a 100-fold reduction is not sufficient and a >1000-fold reduction is required to achieve partial breakdown of GSI. Oxford University Press 2009-03 /pmc/articles/PMC2657546/ /pubmed/19282427 http://dx.doi.org/10.1093/jxb/erp005 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Puerta, Alejandro Raul
Ushijima, Koichiro
Koba, Takato
Sassa, Hidenori
Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title_full Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title_fullStr Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title_full_unstemmed Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title_short Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
title_sort identification and functional analysis of pistil self-incompatibility factor ht-b of petunia
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657546/
https://www.ncbi.nlm.nih.gov/pubmed/19282427
http://dx.doi.org/10.1093/jxb/erp005
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