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Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon

Cross-species transmission of retroviruses is common in Cameroon. To determine risk for simian T-cell lymphotropic virus (STLV) transmission from nonhuman primates to hunters, we examined 170 hunter-collected dried blood spots (DBS) from 12 species for STLV. PCR with generic tax and group-specific l...

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Autores principales: Sintasath, David M., Wolfe, Nathan D., LeBreton, Matthew, Jia, Hongwei, Garcia, Albert D., Diffo, Joseph Le Doux, Tamoufe, Ubald, Carr, Jean K., Folks, Thomas M., Mpoudi-Ngole, Eitel, Burke, Donald S., Heneine, Walid, Switzer, William M.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657614/
https://www.ncbi.nlm.nih.gov/pubmed/19193260
http://dx.doi.org/10.3201/eid1502.080584
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author Sintasath, David M.
Wolfe, Nathan D.
LeBreton, Matthew
Jia, Hongwei
Garcia, Albert D.
Diffo, Joseph Le Doux
Tamoufe, Ubald
Carr, Jean K.
Folks, Thomas M.
Mpoudi-Ngole, Eitel
Burke, Donald S.
Heneine, Walid
Switzer, William M.
author_facet Sintasath, David M.
Wolfe, Nathan D.
LeBreton, Matthew
Jia, Hongwei
Garcia, Albert D.
Diffo, Joseph Le Doux
Tamoufe, Ubald
Carr, Jean K.
Folks, Thomas M.
Mpoudi-Ngole, Eitel
Burke, Donald S.
Heneine, Walid
Switzer, William M.
author_sort Sintasath, David M.
collection PubMed
description Cross-species transmission of retroviruses is common in Cameroon. To determine risk for simian T-cell lymphotropic virus (STLV) transmission from nonhuman primates to hunters, we examined 170 hunter-collected dried blood spots (DBS) from 12 species for STLV. PCR with generic tax and group-specific long terminal repeat primers showed that 12 (7%) specimens from 4 nonhuman primate species were infected with STLV. Phylogenetic analyses showed broad diversity of STLV, including novel STLV-1 and STLV-3 sequences and a highly divergent STLV-3 subtype found in Cercopithecus mona and C. nictitans monkeys. Screening of peripheral blood mononuclear cell DNA from 63 HTLV-seroreactive, PCR-negative hunters did not identify human infections with this divergent STLV-3. Therefore, hunter-collected DBS can effectively capture STLV diversity at the point where pathogen spillover occurs. Broad screening using this relatively easy collection strategy has potential for large-scale monitoring of retrovirus cross-species transmission among highly exposed human populations.
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spelling pubmed-26576142009-03-30 Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon Sintasath, David M. Wolfe, Nathan D. LeBreton, Matthew Jia, Hongwei Garcia, Albert D. Diffo, Joseph Le Doux Tamoufe, Ubald Carr, Jean K. Folks, Thomas M. Mpoudi-Ngole, Eitel Burke, Donald S. Heneine, Walid Switzer, William M. Emerg Infect Dis Research Cross-species transmission of retroviruses is common in Cameroon. To determine risk for simian T-cell lymphotropic virus (STLV) transmission from nonhuman primates to hunters, we examined 170 hunter-collected dried blood spots (DBS) from 12 species for STLV. PCR with generic tax and group-specific long terminal repeat primers showed that 12 (7%) specimens from 4 nonhuman primate species were infected with STLV. Phylogenetic analyses showed broad diversity of STLV, including novel STLV-1 and STLV-3 sequences and a highly divergent STLV-3 subtype found in Cercopithecus mona and C. nictitans monkeys. Screening of peripheral blood mononuclear cell DNA from 63 HTLV-seroreactive, PCR-negative hunters did not identify human infections with this divergent STLV-3. Therefore, hunter-collected DBS can effectively capture STLV diversity at the point where pathogen spillover occurs. Broad screening using this relatively easy collection strategy has potential for large-scale monitoring of retrovirus cross-species transmission among highly exposed human populations. Centers for Disease Control and Prevention 2009-02 /pmc/articles/PMC2657614/ /pubmed/19193260 http://dx.doi.org/10.3201/eid1502.080584 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Sintasath, David M.
Wolfe, Nathan D.
LeBreton, Matthew
Jia, Hongwei
Garcia, Albert D.
Diffo, Joseph Le Doux
Tamoufe, Ubald
Carr, Jean K.
Folks, Thomas M.
Mpoudi-Ngole, Eitel
Burke, Donald S.
Heneine, Walid
Switzer, William M.
Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title_full Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title_fullStr Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title_full_unstemmed Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title_short Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
title_sort simian t-lymphotropic virus diversity among nonhuman primates, cameroon
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657614/
https://www.ncbi.nlm.nih.gov/pubmed/19193260
http://dx.doi.org/10.3201/eid1502.080584
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