Cargando…
Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic fact...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657806/ https://www.ncbi.nlm.nih.gov/pubmed/19216803 http://dx.doi.org/10.1186/1756-9966-28-20 |
_version_ | 1782165618756157440 |
---|---|
author | Kim, Jongmin Hong, Seok Joo Lim, Eun Kyung Yu, Yun-Suk Kim, Seung Whan Roh, Ji Hyeon Do, In-Gu Joh, Jae-Won Kim, Dae Shick |
author_facet | Kim, Jongmin Hong, Seok Joo Lim, Eun Kyung Yu, Yun-Suk Kim, Seung Whan Roh, Ji Hyeon Do, In-Gu Joh, Jae-Won Kim, Dae Shick |
author_sort | Kim, Jongmin |
collection | PubMed |
description | BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic factor. METHODS: Frozen tumors and non-cancerous surrounding tissues from 120 patients with primary HCC were studied. Expressions of NNMT and internal control genes were measured by real-time reverse-transcription PCR (RT-PCR). The relationship of NNMT mRNA level with clinicopathologic parameters and clinical outcome was evaluated. RESULTS: NNMT mRNA level is markedly reduced in HCCs compared to non-cancerous surrounding tissues (P < 0.0001), and NNMT expression in tumors was significantly correlated with tumor stage (P = 0.010). Moreover, stratification of patients based on tumor NNMT mRNA levels revealed that the patients who expressed higher NNMT mRNA levels tended to have a shorter overall survival (OS) time (P = 0.053) and a significantly shorter disease-free survival (DFS) time (P = 0.016). Both NNMT expression (P = 0.0096) and tumor stage (P = 0.0017) were found to be significant prognostic factors for DFS in a multivariate analysis. CONCLUSION: The results of this study indicated that NNMT gene expression is associated with tumor stage and DFS time in HCC cases. Because of the broad substrate specificity of NNMT, which could alter the efficacy and adverse effects of chemotherapy, NNMT merits further investigation regarding its role as a prognostic factor with a larger cohort of HCC patients. |
format | Text |
id | pubmed-2657806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26578062009-03-19 Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis Kim, Jongmin Hong, Seok Joo Lim, Eun Kyung Yu, Yun-Suk Kim, Seung Whan Roh, Ji Hyeon Do, In-Gu Joh, Jae-Won Kim, Dae Shick J Exp Clin Cancer Res Research BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic factor. METHODS: Frozen tumors and non-cancerous surrounding tissues from 120 patients with primary HCC were studied. Expressions of NNMT and internal control genes were measured by real-time reverse-transcription PCR (RT-PCR). The relationship of NNMT mRNA level with clinicopathologic parameters and clinical outcome was evaluated. RESULTS: NNMT mRNA level is markedly reduced in HCCs compared to non-cancerous surrounding tissues (P < 0.0001), and NNMT expression in tumors was significantly correlated with tumor stage (P = 0.010). Moreover, stratification of patients based on tumor NNMT mRNA levels revealed that the patients who expressed higher NNMT mRNA levels tended to have a shorter overall survival (OS) time (P = 0.053) and a significantly shorter disease-free survival (DFS) time (P = 0.016). Both NNMT expression (P = 0.0096) and tumor stage (P = 0.0017) were found to be significant prognostic factors for DFS in a multivariate analysis. CONCLUSION: The results of this study indicated that NNMT gene expression is associated with tumor stage and DFS time in HCC cases. Because of the broad substrate specificity of NNMT, which could alter the efficacy and adverse effects of chemotherapy, NNMT merits further investigation regarding its role as a prognostic factor with a larger cohort of HCC patients. BioMed Central 2009-02-16 /pmc/articles/PMC2657806/ /pubmed/19216803 http://dx.doi.org/10.1186/1756-9966-28-20 Text en Copyright © 2009 Kim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Kim, Jongmin Hong, Seok Joo Lim, Eun Kyung Yu, Yun-Suk Kim, Seung Whan Roh, Ji Hyeon Do, In-Gu Joh, Jae-Won Kim, Dae Shick Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title | Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title_full | Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title_fullStr | Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title_full_unstemmed | Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title_short | Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
title_sort | expression of nicotinamide n-methyltransferase in hepatocellular carcinoma is associated with poor prognosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657806/ https://www.ncbi.nlm.nih.gov/pubmed/19216803 http://dx.doi.org/10.1186/1756-9966-28-20 |
work_keys_str_mv | AT kimjongmin expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT hongseokjoo expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT limeunkyung expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT yuyunsuk expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT kimseungwhan expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT rohjihyeon expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT doingu expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT johjaewon expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis AT kimdaeshick expressionofnicotinamidenmethyltransferaseinhepatocellularcarcinomaisassociatedwithpoorprognosis |