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Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis

BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic fact...

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Autores principales: Kim, Jongmin, Hong, Seok Joo, Lim, Eun Kyung, Yu, Yun-Suk, Kim, Seung Whan, Roh, Ji Hyeon, Do, In-Gu, Joh, Jae-Won, Kim, Dae Shick
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657806/
https://www.ncbi.nlm.nih.gov/pubmed/19216803
http://dx.doi.org/10.1186/1756-9966-28-20
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author Kim, Jongmin
Hong, Seok Joo
Lim, Eun Kyung
Yu, Yun-Suk
Kim, Seung Whan
Roh, Ji Hyeon
Do, In-Gu
Joh, Jae-Won
Kim, Dae Shick
author_facet Kim, Jongmin
Hong, Seok Joo
Lim, Eun Kyung
Yu, Yun-Suk
Kim, Seung Whan
Roh, Ji Hyeon
Do, In-Gu
Joh, Jae-Won
Kim, Dae Shick
author_sort Kim, Jongmin
collection PubMed
description BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic factor. METHODS: Frozen tumors and non-cancerous surrounding tissues from 120 patients with primary HCC were studied. Expressions of NNMT and internal control genes were measured by real-time reverse-transcription PCR (RT-PCR). The relationship of NNMT mRNA level with clinicopathologic parameters and clinical outcome was evaluated. RESULTS: NNMT mRNA level is markedly reduced in HCCs compared to non-cancerous surrounding tissues (P < 0.0001), and NNMT expression in tumors was significantly correlated with tumor stage (P = 0.010). Moreover, stratification of patients based on tumor NNMT mRNA levels revealed that the patients who expressed higher NNMT mRNA levels tended to have a shorter overall survival (OS) time (P = 0.053) and a significantly shorter disease-free survival (DFS) time (P = 0.016). Both NNMT expression (P = 0.0096) and tumor stage (P = 0.0017) were found to be significant prognostic factors for DFS in a multivariate analysis. CONCLUSION: The results of this study indicated that NNMT gene expression is associated with tumor stage and DFS time in HCC cases. Because of the broad substrate specificity of NNMT, which could alter the efficacy and adverse effects of chemotherapy, NNMT merits further investigation regarding its role as a prognostic factor with a larger cohort of HCC patients.
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spelling pubmed-26578062009-03-19 Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis Kim, Jongmin Hong, Seok Joo Lim, Eun Kyung Yu, Yun-Suk Kim, Seung Whan Roh, Ji Hyeon Do, In-Gu Joh, Jae-Won Kim, Dae Shick J Exp Clin Cancer Res Research BACKGROUND: Hepatocellular carcinoma (HCC) is the most common tumor in the adult liver, with high relapse and mortality rates despite diverse treatment modalities. In this study, nicotinamide N-methyltransferase (NNMT), a key enzyme in drug metabolism, was investigated as a potential prognostic factor. METHODS: Frozen tumors and non-cancerous surrounding tissues from 120 patients with primary HCC were studied. Expressions of NNMT and internal control genes were measured by real-time reverse-transcription PCR (RT-PCR). The relationship of NNMT mRNA level with clinicopathologic parameters and clinical outcome was evaluated. RESULTS: NNMT mRNA level is markedly reduced in HCCs compared to non-cancerous surrounding tissues (P < 0.0001), and NNMT expression in tumors was significantly correlated with tumor stage (P = 0.010). Moreover, stratification of patients based on tumor NNMT mRNA levels revealed that the patients who expressed higher NNMT mRNA levels tended to have a shorter overall survival (OS) time (P = 0.053) and a significantly shorter disease-free survival (DFS) time (P = 0.016). Both NNMT expression (P = 0.0096) and tumor stage (P = 0.0017) were found to be significant prognostic factors for DFS in a multivariate analysis. CONCLUSION: The results of this study indicated that NNMT gene expression is associated with tumor stage and DFS time in HCC cases. Because of the broad substrate specificity of NNMT, which could alter the efficacy and adverse effects of chemotherapy, NNMT merits further investigation regarding its role as a prognostic factor with a larger cohort of HCC patients. BioMed Central 2009-02-16 /pmc/articles/PMC2657806/ /pubmed/19216803 http://dx.doi.org/10.1186/1756-9966-28-20 Text en Copyright © 2009 Kim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kim, Jongmin
Hong, Seok Joo
Lim, Eun Kyung
Yu, Yun-Suk
Kim, Seung Whan
Roh, Ji Hyeon
Do, In-Gu
Joh, Jae-Won
Kim, Dae Shick
Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title_full Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title_fullStr Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title_full_unstemmed Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title_short Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
title_sort expression of nicotinamide n-methyltransferase in hepatocellular carcinoma is associated with poor prognosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657806/
https://www.ncbi.nlm.nih.gov/pubmed/19216803
http://dx.doi.org/10.1186/1756-9966-28-20
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