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Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors

BACKGROUND: Although features of variable differentiation in glioblastoma cell cultures have been reported, a comparative analysis of differentiation properties of normal neural GFAP positive progenitors, and those shown by glioblastoma cells, has not been performed. METHODS: Following methods were...

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Autores principales: Rieske, Piotr, Golanska, Ewa, Zakrzewska, Magdalena, Piaskowski, Sylwester, Hulas-Bigoszewska, Krystyna, Wolańczyk, Magdalena, Szybka, Malgorzata, Witusik-Perkowska, Monika, Jaskolski, Dariusz J, Zakrzewski, Krzysztof, Biernat, Wojciech, Krynska, Barbara, Liberski, Pawel P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657909/
https://www.ncbi.nlm.nih.gov/pubmed/19216795
http://dx.doi.org/10.1186/1471-2407-9-54
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author Rieske, Piotr
Golanska, Ewa
Zakrzewska, Magdalena
Piaskowski, Sylwester
Hulas-Bigoszewska, Krystyna
Wolańczyk, Magdalena
Szybka, Malgorzata
Witusik-Perkowska, Monika
Jaskolski, Dariusz J
Zakrzewski, Krzysztof
Biernat, Wojciech
Krynska, Barbara
Liberski, Pawel P
author_facet Rieske, Piotr
Golanska, Ewa
Zakrzewska, Magdalena
Piaskowski, Sylwester
Hulas-Bigoszewska, Krystyna
Wolańczyk, Magdalena
Szybka, Malgorzata
Witusik-Perkowska, Monika
Jaskolski, Dariusz J
Zakrzewski, Krzysztof
Biernat, Wojciech
Krynska, Barbara
Liberski, Pawel P
author_sort Rieske, Piotr
collection PubMed
description BACKGROUND: Although features of variable differentiation in glioblastoma cell cultures have been reported, a comparative analysis of differentiation properties of normal neural GFAP positive progenitors, and those shown by glioblastoma cells, has not been performed. METHODS: Following methods were used to compare glioblastoma cells and GFAP+NNP (NHA): exposure to neural differentiation medium, exposure to adipogenic and osteogenic medium, western blot analysis, immunocytochemistry, single cell assay, BrdU incorporation assay. To characterize glioblastoma cells EGFR amplification analysis, LOH/MSI analysis, and P53 nucleotide sequence analysis were performed. RESULTS: In vitro differentiation of cancer cells derived from eight glioblastomas was compared with GFAP-positive normal neural progenitors (GFAP+NNP). Prior to exposure to differentiation medium, both types of cells showed similar multilineage phenotype (CD44+/MAP2+/GFAP+/Vimentin+/Beta III-tubulin+/Fibronectin+) and were positive for SOX-2 and Nestin. In contrast to GFAP+NNP, an efficient differentiation arrest was observed in all cell lines isolated from glioblastomas. Nevertheless, a subpopulation of cells isolated from four glioblastomas differentiated after serum-starvation with varying efficiency into derivatives indistinguishable from the neural derivatives of GFAP+NNP. Moreover, the cells derived from a majority of glioblastomas (7 out of 8), as well as GFAP+NNP, showed features of mesenchymal differentiation when exposed to medium with serum. CONCLUSION: Our results showed that stable co-expression of multilineage markers by glioblastoma cells resulted from differentiation arrest. According to our data up to 95% of glioblastoma cells can present in vitro multilineage phenotype. The mesenchymal differentiation of glioblastoma cells is advanced and similar to mesenchymal differentiation of normal neural progenitors GFAP+NNP.
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spelling pubmed-26579092009-03-20 Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors Rieske, Piotr Golanska, Ewa Zakrzewska, Magdalena Piaskowski, Sylwester Hulas-Bigoszewska, Krystyna Wolańczyk, Magdalena Szybka, Malgorzata Witusik-Perkowska, Monika Jaskolski, Dariusz J Zakrzewski, Krzysztof Biernat, Wojciech Krynska, Barbara Liberski, Pawel P BMC Cancer Research Article BACKGROUND: Although features of variable differentiation in glioblastoma cell cultures have been reported, a comparative analysis of differentiation properties of normal neural GFAP positive progenitors, and those shown by glioblastoma cells, has not been performed. METHODS: Following methods were used to compare glioblastoma cells and GFAP+NNP (NHA): exposure to neural differentiation medium, exposure to adipogenic and osteogenic medium, western blot analysis, immunocytochemistry, single cell assay, BrdU incorporation assay. To characterize glioblastoma cells EGFR amplification analysis, LOH/MSI analysis, and P53 nucleotide sequence analysis were performed. RESULTS: In vitro differentiation of cancer cells derived from eight glioblastomas was compared with GFAP-positive normal neural progenitors (GFAP+NNP). Prior to exposure to differentiation medium, both types of cells showed similar multilineage phenotype (CD44+/MAP2+/GFAP+/Vimentin+/Beta III-tubulin+/Fibronectin+) and were positive for SOX-2 and Nestin. In contrast to GFAP+NNP, an efficient differentiation arrest was observed in all cell lines isolated from glioblastomas. Nevertheless, a subpopulation of cells isolated from four glioblastomas differentiated after serum-starvation with varying efficiency into derivatives indistinguishable from the neural derivatives of GFAP+NNP. Moreover, the cells derived from a majority of glioblastomas (7 out of 8), as well as GFAP+NNP, showed features of mesenchymal differentiation when exposed to medium with serum. CONCLUSION: Our results showed that stable co-expression of multilineage markers by glioblastoma cells resulted from differentiation arrest. According to our data up to 95% of glioblastoma cells can present in vitro multilineage phenotype. The mesenchymal differentiation of glioblastoma cells is advanced and similar to mesenchymal differentiation of normal neural progenitors GFAP+NNP. BioMed Central 2009-02-14 /pmc/articles/PMC2657909/ /pubmed/19216795 http://dx.doi.org/10.1186/1471-2407-9-54 Text en Copyright ©2009 Rieske et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rieske, Piotr
Golanska, Ewa
Zakrzewska, Magdalena
Piaskowski, Sylwester
Hulas-Bigoszewska, Krystyna
Wolańczyk, Magdalena
Szybka, Malgorzata
Witusik-Perkowska, Monika
Jaskolski, Dariusz J
Zakrzewski, Krzysztof
Biernat, Wojciech
Krynska, Barbara
Liberski, Pawel P
Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title_full Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title_fullStr Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title_full_unstemmed Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title_short Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
title_sort arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657909/
https://www.ncbi.nlm.nih.gov/pubmed/19216795
http://dx.doi.org/10.1186/1471-2407-9-54
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