Nodal signaling is involved in left-right asymmetry in snails

Many animals display specific internal or external features with left-right asymmetry. In vertebrates, the molecular pathway that leads to this asymmetry utilizes the signaling molecule Nodal, a member of the TGF-β superfamily 1, that is expressed in the left lateral plate mesoderm 2, and loss of nodal function produces a randomization of the left-right asymmetry of visceral organs 3,4. Orthologs of nodal have also been described in other deuterostomes, including ascidians and sea urchins 5-6, but no nodal ortholog has been reported in the other two main clades of Bilateria: Ecdysozoa (including flies and nematodes) and Lophotrochozoa (including snails and annelids). Here we report the first evidence for a nodal ortholog in a non-deuterostome group. We isolated nodal and Pitx (one of the targets of Nodal signaling) in two species of snails and found that the side of the embryo that expresses nodal and Pitx is related to body chirality: both genes are expressed on the right side of the embryo in the dextral (right handed) species Lottia gigantea and on the left side in the sinistral (left handed) species Biomphalaria glabrata. We pharmacologically inhibited the Nodal pathway and found that nodal acts upstream of Pitx, and that some treated animals developed with a loss of shell chirality. These results suggest that the involvement of the Nodal pathway in left-right asymmetry might have been an ancestral feature of the Bilateria.