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In Situ Proteolysis to Generate Crystals for Structure Determination: An Update
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13–15 crystallize in a form that enables structure determination. In 2007, Dong et al reported that the addition of trace amounts of protease to crystallization trials—in situ prot...
Autores principales: | Wernimont, Amy, Edwards, Aled |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2661377/ https://www.ncbi.nlm.nih.gov/pubmed/19352432 http://dx.doi.org/10.1371/journal.pone.0005094 |
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