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Over-expression, Rapid Preparation and Some Properties of C-terminal BARc Region in PICK1

A DNA fragment encoding C-terminal BARc region (amino acids 128–416) of rat PICK1 (NP_445912 ) was inserted into a modified vector pMAL-s involving human rhinovirus 3C protease cleavage site to produce a recombinant plasmid, pMAL-s-barc. The construct can express the fusion protein, MBP-BARc in the...

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Detalles Bibliográficos
Autores principales: Xiao, Hong, Shi, Yawei, Yuan, Jingming, Huang, Yuming, Wang, Junhua
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662470/
https://www.ncbi.nlm.nih.gov/pubmed/19333433
http://dx.doi.org/10.3390/ijms10010028