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Over-expression, Rapid Preparation and Some Properties of C-terminal BARc Region in PICK1
A DNA fragment encoding C-terminal BARc region (amino acids 128–416) of rat PICK1 (NP_445912 ) was inserted into a modified vector pMAL-s involving human rhinovirus 3C protease cleavage site to produce a recombinant plasmid, pMAL-s-barc. The construct can express the fusion protein, MBP-BARc in the...
Autores principales: | Xiao, Hong, Shi, Yawei, Yuan, Jingming, Huang, Yuming, Wang, Junhua |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662470/ https://www.ncbi.nlm.nih.gov/pubmed/19333433 http://dx.doi.org/10.3390/ijms10010028 |
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