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Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain

In low nanomolar concentrations, NO (nitric oxide) functions as a transmitter in brain and other tissues, whereas near-micromolar NO concentrations are associated with toxicity and cell death. Control of the NO concentration, therefore, is critical for proper brain function, but, although its synthe...

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Autores principales: Hall, Catherine N., Keynes, Robert G., Garthwaite, John
Formato: Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662488/
https://www.ncbi.nlm.nih.gov/pubmed/19152507
http://dx.doi.org/10.1042/BJ20082419
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author Hall, Catherine N.
Keynes, Robert G.
Garthwaite, John
author_facet Hall, Catherine N.
Keynes, Robert G.
Garthwaite, John
author_sort Hall, Catherine N.
collection PubMed
description In low nanomolar concentrations, NO (nitric oxide) functions as a transmitter in brain and other tissues, whereas near-micromolar NO concentrations are associated with toxicity and cell death. Control of the NO concentration, therefore, is critical for proper brain function, but, although its synthesis pathway is well-characterized, the major route of breakdown of NO in brain is unclear. Previous observations indicate that brain cells actively consume NO at a high rate. The mechanism of this consumption was pursued in the present study. NO consumption by a preparation of central glial cells was abolished by cell lysis and recovered by addition of NADPH. NADPH-dependent consumption of NO localized to cell membranes and was inhibited by proteinase K, indicating the involvement of a membrane-bound protein. Purification of this activity yielded CYPOR (cytochrome P450 oxidoreductase). Antibodies against CYPOR inhibited NO consumption by brain membranes and the amount of CYPOR in several cell types correlated with their rate of NO consumption. NO was also consumed by purified CYPOR but this activity was found to depend on the presence of the vitamin E analogue Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), included in the buffer as a precaution against inadvertent NO consumption by lipid peroxidation. In contrast, NO consumption by brain membranes was independent of Trolox. Hence, it appears that, during the purification process, CYPOR becomes separated from a partner needed for NO consumption. Cytochrome P450 inhibitors inhibited NO consumption by brain membranes, making these proteins likely candidates.
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spelling pubmed-26624882009-03-30 Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain Hall, Catherine N. Keynes, Robert G. Garthwaite, John Biochem J Research Article In low nanomolar concentrations, NO (nitric oxide) functions as a transmitter in brain and other tissues, whereas near-micromolar NO concentrations are associated with toxicity and cell death. Control of the NO concentration, therefore, is critical for proper brain function, but, although its synthesis pathway is well-characterized, the major route of breakdown of NO in brain is unclear. Previous observations indicate that brain cells actively consume NO at a high rate. The mechanism of this consumption was pursued in the present study. NO consumption by a preparation of central glial cells was abolished by cell lysis and recovered by addition of NADPH. NADPH-dependent consumption of NO localized to cell membranes and was inhibited by proteinase K, indicating the involvement of a membrane-bound protein. Purification of this activity yielded CYPOR (cytochrome P450 oxidoreductase). Antibodies against CYPOR inhibited NO consumption by brain membranes and the amount of CYPOR in several cell types correlated with their rate of NO consumption. NO was also consumed by purified CYPOR but this activity was found to depend on the presence of the vitamin E analogue Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), included in the buffer as a precaution against inadvertent NO consumption by lipid peroxidation. In contrast, NO consumption by brain membranes was independent of Trolox. Hence, it appears that, during the purification process, CYPOR becomes separated from a partner needed for NO consumption. Cytochrome P450 inhibitors inhibited NO consumption by brain membranes, making these proteins likely candidates. Portland Press Ltd. 2009-03-27 2009-04-15 /pmc/articles/PMC2662488/ /pubmed/19152507 http://dx.doi.org/10.1042/BJ20082419 Text en © 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hall, Catherine N.
Keynes, Robert G.
Garthwaite, John
Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title_full Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title_fullStr Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title_full_unstemmed Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title_short Cytochrome P450 oxidoreductase participates in nitric oxide consumption by rat brain
title_sort cytochrome p450 oxidoreductase participates in nitric oxide consumption by rat brain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662488/
https://www.ncbi.nlm.nih.gov/pubmed/19152507
http://dx.doi.org/10.1042/BJ20082419
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