Cargando…

Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor

Bluetongue (BT) is a noncontagious, insect-transmitted disease of ruminants caused by the bluetongue virus (BTV). A laboratory animal model would greatly facilitate the studies of pathogenesis, immune response and vaccination against BTV. Herein, we show that adult mice deficient in type I IFN recep...

Descripción completa

Detalles Bibliográficos
Autores principales: Calvo-Pinilla, Eva, Rodríguez-Calvo, Teresa, Anguita, Juan, Sevilla, Noemí, Ortego, Javier
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663843/
https://www.ncbi.nlm.nih.gov/pubmed/19357779
http://dx.doi.org/10.1371/journal.pone.0005171
_version_ 1782165928094466048
author Calvo-Pinilla, Eva
Rodríguez-Calvo, Teresa
Anguita, Juan
Sevilla, Noemí
Ortego, Javier
author_facet Calvo-Pinilla, Eva
Rodríguez-Calvo, Teresa
Anguita, Juan
Sevilla, Noemí
Ortego, Javier
author_sort Calvo-Pinilla, Eva
collection PubMed
description Bluetongue (BT) is a noncontagious, insect-transmitted disease of ruminants caused by the bluetongue virus (BTV). A laboratory animal model would greatly facilitate the studies of pathogenesis, immune response and vaccination against BTV. Herein, we show that adult mice deficient in type I IFN receptor (IFNAR((−/−))) are highly susceptible to BTV-4 and BTV-8 infection when the virus is administered intravenously. Disease was characterized by ocular discharges and apathy, starting at 48 hours post-infection and quickly leading to animal death within 60 hours of inoculation. Infectious virus was recovered from the spleen, lung, thymus, and lymph nodes indicating a systemic infection. In addition, a lymphoid depletion in spleen, and severe pneumonia were observed in the infected mice. Furthermore, IFNAR((−/−)) adult mice immunized with a BTV-4 inactivated vaccine showed the induction of neutralizing antibodies against BTV-4 and complete protection against challenge with a lethal dose of this virus. The data indicate that this mouse model may facilitate the study of BTV pathogenesis, and the development of new effective vaccines for BTV.
format Text
id pubmed-2663843
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-26638432009-04-09 Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor Calvo-Pinilla, Eva Rodríguez-Calvo, Teresa Anguita, Juan Sevilla, Noemí Ortego, Javier PLoS One Research Article Bluetongue (BT) is a noncontagious, insect-transmitted disease of ruminants caused by the bluetongue virus (BTV). A laboratory animal model would greatly facilitate the studies of pathogenesis, immune response and vaccination against BTV. Herein, we show that adult mice deficient in type I IFN receptor (IFNAR((−/−))) are highly susceptible to BTV-4 and BTV-8 infection when the virus is administered intravenously. Disease was characterized by ocular discharges and apathy, starting at 48 hours post-infection and quickly leading to animal death within 60 hours of inoculation. Infectious virus was recovered from the spleen, lung, thymus, and lymph nodes indicating a systemic infection. In addition, a lymphoid depletion in spleen, and severe pneumonia were observed in the infected mice. Furthermore, IFNAR((−/−)) adult mice immunized with a BTV-4 inactivated vaccine showed the induction of neutralizing antibodies against BTV-4 and complete protection against challenge with a lethal dose of this virus. The data indicate that this mouse model may facilitate the study of BTV pathogenesis, and the development of new effective vaccines for BTV. Public Library of Science 2009-04-09 /pmc/articles/PMC2663843/ /pubmed/19357779 http://dx.doi.org/10.1371/journal.pone.0005171 Text en Calvo-Pinilla et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Calvo-Pinilla, Eva
Rodríguez-Calvo, Teresa
Anguita, Juan
Sevilla, Noemí
Ortego, Javier
Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title_full Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title_fullStr Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title_full_unstemmed Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title_short Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor
title_sort establishment of a bluetongue virus infection model in mice that are deficient in the alpha/beta interferon receptor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663843/
https://www.ncbi.nlm.nih.gov/pubmed/19357779
http://dx.doi.org/10.1371/journal.pone.0005171
work_keys_str_mv AT calvopinillaeva establishmentofabluetonguevirusinfectionmodelinmicethataredeficientinthealphabetainterferonreceptor
AT rodriguezcalvoteresa establishmentofabluetonguevirusinfectionmodelinmicethataredeficientinthealphabetainterferonreceptor
AT anguitajuan establishmentofabluetonguevirusinfectionmodelinmicethataredeficientinthealphabetainterferonreceptor
AT sevillanoemi establishmentofabluetonguevirusinfectionmodelinmicethataredeficientinthealphabetainterferonreceptor
AT ortegojavier establishmentofabluetonguevirusinfectionmodelinmicethataredeficientinthealphabetainterferonreceptor