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Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans

[Image: see text] Homalanthus nutans, used by Samoan healers to treat hepatitis, produces the antiviral compound 12-deoxyphorbol 13-acetate, prostratin (1). Prostratin is being developed as an adjuvant therapy to clear latent viral reservoirs, the major obstacle to eradication of HIV-AIDS within the...

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Autores principales: Johnson, Holly E., Banack, Sandra A., Cox, Paul Alan
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society and American Society of Pharmacognosy 2008
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663895/
https://www.ncbi.nlm.nih.gov/pubmed/19007283
http://dx.doi.org/10.1021/np800295m
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author Johnson, Holly E.
Banack, Sandra A.
Cox, Paul Alan
author_facet Johnson, Holly E.
Banack, Sandra A.
Cox, Paul Alan
author_sort Johnson, Holly E.
collection PubMed
description [Image: see text] Homalanthus nutans, used by Samoan healers to treat hepatitis, produces the antiviral compound 12-deoxyphorbol 13-acetate, prostratin (1). Prostratin is being developed as an adjuvant therapy to clear latent viral reservoirs, the major obstacle to eradication of HIV-AIDS within the human body. A validated reversed-phase HPLC method was developed to assay concentrations of 1 in H. nutans. A survey of four distinct populations on two different Samoan islands revealed significant variability in content. The stem tissue (range 0.2−52.6 μg/g 1), used by healers in indigenous therapies, gave a higher median concentration of prostratin (3.5 μg/g) than root or leaf tissues (2.9 and 2.5 μg/g, respectively). The high variability and skewness of these data indicate that cultivar selection for drug production will be important for this species. The reversed-phase HPLC assay will allow plants to be selected for agricultural development and genetic analysis by identifying those individuals above and below a 95% confidence interval for the median concentration.
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spelling pubmed-26638952009-04-02 Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans Johnson, Holly E. Banack, Sandra A. Cox, Paul Alan J Nat Prod [Image: see text] Homalanthus nutans, used by Samoan healers to treat hepatitis, produces the antiviral compound 12-deoxyphorbol 13-acetate, prostratin (1). Prostratin is being developed as an adjuvant therapy to clear latent viral reservoirs, the major obstacle to eradication of HIV-AIDS within the human body. A validated reversed-phase HPLC method was developed to assay concentrations of 1 in H. nutans. A survey of four distinct populations on two different Samoan islands revealed significant variability in content. The stem tissue (range 0.2−52.6 μg/g 1), used by healers in indigenous therapies, gave a higher median concentration of prostratin (3.5 μg/g) than root or leaf tissues (2.9 and 2.5 μg/g, respectively). The high variability and skewness of these data indicate that cultivar selection for drug production will be important for this species. The reversed-phase HPLC assay will allow plants to be selected for agricultural development and genetic analysis by identifying those individuals above and below a 95% confidence interval for the median concentration. American Chemical Society and American Society of Pharmacognosy 2008-11-14 2008-12-26 /pmc/articles/PMC2663895/ /pubmed/19007283 http://dx.doi.org/10.1021/np800295m Text en Copyright © 2008 American Chemical Society and American Society of Pharmacognosy http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org. 40.75
spellingShingle Johnson, Holly E.
Banack, Sandra A.
Cox, Paul Alan
Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title_full Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title_fullStr Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title_full_unstemmed Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title_short Variability in Content of the Anti-AIDS Drug Candidate Prostratin in Samoan Populations of Homalanthus nutans
title_sort variability in content of the anti-aids drug candidate prostratin in samoan populations of homalanthus nutans
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663895/
https://www.ncbi.nlm.nih.gov/pubmed/19007283
http://dx.doi.org/10.1021/np800295m
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