Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to...

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Autores principales: Ohtsuka, Masato, Warita, Takayuki, Sakurai, Takayuki, Watanabe, Satoshi, Inoko, Hidetoshi, Sato, Masahiro
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664460/
https://www.ncbi.nlm.nih.gov/pubmed/19360101
http://dx.doi.org/10.1155/2009/985140
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author Ohtsuka, Masato
Warita, Takayuki
Sakurai, Takayuki
Watanabe, Satoshi
Inoko, Hidetoshi
Sato, Masahiro
author_facet Ohtsuka, Masato
Warita, Takayuki
Sakurai, Takayuki
Watanabe, Satoshi
Inoko, Hidetoshi
Sato, Masahiro
author_sort Ohtsuka, Masato
collection PubMed
description We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion.
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spelling pubmed-26644602009-04-09 Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector Ohtsuka, Masato Warita, Takayuki Sakurai, Takayuki Watanabe, Satoshi Inoko, Hidetoshi Sato, Masahiro J Biomed Biotechnol Research Article We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion. Hindawi Publishing Corporation 2009 2009-04-01 /pmc/articles/PMC2664460/ /pubmed/19360101 http://dx.doi.org/10.1155/2009/985140 Text en Copyright © 2009 Masato Ohtsuka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ohtsuka, Masato
Warita, Takayuki
Sakurai, Takayuki
Watanabe, Satoshi
Inoko, Hidetoshi
Sato, Masahiro
Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title_full Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title_fullStr Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title_full_unstemmed Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title_short Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
title_sort development of crteil and cetriz, cre-loxp-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664460/
https://www.ncbi.nlm.nih.gov/pubmed/19360101
http://dx.doi.org/10.1155/2009/985140
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