Cargando…
The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and con...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664462/ https://www.ncbi.nlm.nih.gov/pubmed/19357783 http://dx.doi.org/10.1371/journal.pone.0005142 |
_version_ | 1782165969688330240 |
---|---|
author | Gorski, Lisa Duhé, Jessica M. Flaherty, Denise |
author_facet | Gorski, Lisa Duhé, Jessica M. Flaherty, Denise |
author_sort | Gorski, Lisa |
collection | PubMed |
description | The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either flaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the flaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a flaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the flaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases. |
format | Text |
id | pubmed-2664462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26644622009-04-09 The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes Gorski, Lisa Duhé, Jessica M. Flaherty, Denise PLoS One Research Article The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either flaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the flaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a flaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the flaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases. Public Library of Science 2009-04-09 /pmc/articles/PMC2664462/ /pubmed/19357783 http://dx.doi.org/10.1371/journal.pone.0005142 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Gorski, Lisa Duhé, Jessica M. Flaherty, Denise The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes |
title | The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
|
title_full | The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
|
title_fullStr | The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
|
title_full_unstemmed | The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
|
title_short | The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes
|
title_sort | use of flagella and motility for plant colonization and fitness by different strains of the foodborne pathogen listeria monocytogenes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664462/ https://www.ncbi.nlm.nih.gov/pubmed/19357783 http://dx.doi.org/10.1371/journal.pone.0005142 |
work_keys_str_mv | AT gorskilisa theuseofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes AT duhejessicam theuseofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes AT flahertydenise theuseofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes AT gorskilisa useofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes AT duhejessicam useofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes AT flahertydenise useofflagellaandmotilityforplantcolonizationandfitnessbydifferentstrainsofthefoodbornepathogenlisteriamonocytogenes |