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Comparison of bee products based on assays of antioxidant capacities

BACKGROUND: Bee products (including propolis, royal jelly, and bee pollen) are popular, traditional health foods. We compared antioxidant effects among water and ethanol extracts of Brazilian green propolis (WEP or EEP), its main constituents, water-soluble royal jelly (RJ), and an ethanol extract o...

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Autores principales: Nakajima, Yoshimi, Tsuruma, Kazuhiro, Shimazawa, Masamitsu, Mishima, Satoshi, Hara, Hideaki
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664783/
https://www.ncbi.nlm.nih.gov/pubmed/19243635
http://dx.doi.org/10.1186/1472-6882-9-4
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author Nakajima, Yoshimi
Tsuruma, Kazuhiro
Shimazawa, Masamitsu
Mishima, Satoshi
Hara, Hideaki
author_facet Nakajima, Yoshimi
Tsuruma, Kazuhiro
Shimazawa, Masamitsu
Mishima, Satoshi
Hara, Hideaki
author_sort Nakajima, Yoshimi
collection PubMed
description BACKGROUND: Bee products (including propolis, royal jelly, and bee pollen) are popular, traditional health foods. We compared antioxidant effects among water and ethanol extracts of Brazilian green propolis (WEP or EEP), its main constituents, water-soluble royal jelly (RJ), and an ethanol extract of bee pollen. METHODS: The hydrogen peroxide (H(2)O(2))-, superoxide anion (O(2)(·-))-, and hydroxyl radical (HO(·))- scavenging capacities of bee products were measured using antioxidant capacity assays that employed the reactive oxygen species (ROS)-sensitive probe 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H(2)DCFDA) or aminophenyl fluorescein (APF). RESULTS: The rank order of antioxidant potencies was as follows: WEP > EEP > pollen, but neither RJ nor 10-hydroxy-2-decenoic acid (10-HDA) had any effects. Concerning the main constituents of WEP, the rank order of antioxidant effects was: caffeic acid > artepillin C > drupanin, but neither baccharin nor coumaric acid had any effects. The scavenging effects of caffeic acid were as powerful as those of trolox, but stronger than those of N-acetyl cysteine (NAC) or vitamin C. CONCLUSION: On the basis of the present assays, propolis is the most powerful antioxidant of all the bee product examined, and its effect may be partly due to the various caffeic acids it contains. Pollen, too, exhibited strong antioxidant effects.
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spelling pubmed-26647832009-04-03 Comparison of bee products based on assays of antioxidant capacities Nakajima, Yoshimi Tsuruma, Kazuhiro Shimazawa, Masamitsu Mishima, Satoshi Hara, Hideaki BMC Complement Altern Med Research Article BACKGROUND: Bee products (including propolis, royal jelly, and bee pollen) are popular, traditional health foods. We compared antioxidant effects among water and ethanol extracts of Brazilian green propolis (WEP or EEP), its main constituents, water-soluble royal jelly (RJ), and an ethanol extract of bee pollen. METHODS: The hydrogen peroxide (H(2)O(2))-, superoxide anion (O(2)(·-))-, and hydroxyl radical (HO(·))- scavenging capacities of bee products were measured using antioxidant capacity assays that employed the reactive oxygen species (ROS)-sensitive probe 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H(2)DCFDA) or aminophenyl fluorescein (APF). RESULTS: The rank order of antioxidant potencies was as follows: WEP > EEP > pollen, but neither RJ nor 10-hydroxy-2-decenoic acid (10-HDA) had any effects. Concerning the main constituents of WEP, the rank order of antioxidant effects was: caffeic acid > artepillin C > drupanin, but neither baccharin nor coumaric acid had any effects. The scavenging effects of caffeic acid were as powerful as those of trolox, but stronger than those of N-acetyl cysteine (NAC) or vitamin C. CONCLUSION: On the basis of the present assays, propolis is the most powerful antioxidant of all the bee product examined, and its effect may be partly due to the various caffeic acids it contains. Pollen, too, exhibited strong antioxidant effects. BioMed Central 2009-02-26 /pmc/articles/PMC2664783/ /pubmed/19243635 http://dx.doi.org/10.1186/1472-6882-9-4 Text en Copyright © 2009 Nakajima et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nakajima, Yoshimi
Tsuruma, Kazuhiro
Shimazawa, Masamitsu
Mishima, Satoshi
Hara, Hideaki
Comparison of bee products based on assays of antioxidant capacities
title Comparison of bee products based on assays of antioxidant capacities
title_full Comparison of bee products based on assays of antioxidant capacities
title_fullStr Comparison of bee products based on assays of antioxidant capacities
title_full_unstemmed Comparison of bee products based on assays of antioxidant capacities
title_short Comparison of bee products based on assays of antioxidant capacities
title_sort comparison of bee products based on assays of antioxidant capacities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664783/
https://www.ncbi.nlm.nih.gov/pubmed/19243635
http://dx.doi.org/10.1186/1472-6882-9-4
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