Cargando…
Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs†
[Image: see text] G-Protein-coupled receptors (GPCRs) adopt various functionally relevant conformational states in cell signaling processes. Recently determined crystal structures of rhodopsin and the β(2)-adrenergic receptor (β(2)-AR) offer insight into previously uncharacterized active conformatio...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2008
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664832/ https://www.ncbi.nlm.nih.gov/pubmed/18558776 http://dx.doi.org/10.1021/bi800442g |
_version_ | 1782165997803798528 |
---|---|
author | Han, Daniel S. Wang, Simon X. Weinstein, Harel |
author_facet | Han, Daniel S. Wang, Simon X. Weinstein, Harel |
author_sort | Han, Daniel S. |
collection | PubMed |
description | [Image: see text] G-Protein-coupled receptors (GPCRs) adopt various functionally relevant conformational states in cell signaling processes. Recently determined crystal structures of rhodopsin and the β(2)-adrenergic receptor (β(2)-AR) offer insight into previously uncharacterized active conformations, but the molecular states of these GPCRs are likely to contain both inactive and active-like conformational elements. We have identified conformational rearrangements in the dynamics of the TM7−HX8 segment that relate to the properties of the conserved NPxxY(x)5,6F motif and show that they can be used to identify active state-like conformational elements in the corresponding regions of the new structures of rhodopsin and the β(2)-AR. |
format | Text |
id | pubmed-2664832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-26648322009-04-06 Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† Han, Daniel S. Wang, Simon X. Weinstein, Harel Biochemistry [Image: see text] G-Protein-coupled receptors (GPCRs) adopt various functionally relevant conformational states in cell signaling processes. Recently determined crystal structures of rhodopsin and the β(2)-adrenergic receptor (β(2)-AR) offer insight into previously uncharacterized active conformations, but the molecular states of these GPCRs are likely to contain both inactive and active-like conformational elements. We have identified conformational rearrangements in the dynamics of the TM7−HX8 segment that relate to the properties of the conserved NPxxY(x)5,6F motif and show that they can be used to identify active state-like conformational elements in the corresponding regions of the new structures of rhodopsin and the β(2)-AR. American Chemical Society 2008-06-18 2008-07-15 /pmc/articles/PMC2664832/ /pubmed/18558776 http://dx.doi.org/10.1021/bi800442g Text en Copyright © 2008 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org. 40.75 |
spellingShingle | Han, Daniel S. Wang, Simon X. Weinstein, Harel Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title | Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title_full | Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title_fullStr | Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title_full_unstemmed | Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title_short | Active State-like Conformational Elements in the β(2)-AR and a Photoactivated Intermediate of Rhodopsin Identified by Dynamic Properties of GPCRs† |
title_sort | active state-like conformational elements in the β(2)-ar and a photoactivated intermediate of rhodopsin identified by dynamic properties of gpcrs† |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664832/ https://www.ncbi.nlm.nih.gov/pubmed/18558776 http://dx.doi.org/10.1021/bi800442g |
work_keys_str_mv | AT handaniels activestatelikeconformationalelementsintheb2arandaphotoactivatedintermediateofrhodopsinidentifiedbydynamicpropertiesofgpcrs AT wangsimonx activestatelikeconformationalelementsintheb2arandaphotoactivatedintermediateofrhodopsinidentifiedbydynamicpropertiesofgpcrs AT weinsteinharel activestatelikeconformationalelementsintheb2arandaphotoactivatedintermediateofrhodopsinidentifiedbydynamicpropertiesofgpcrs |