Label-free electrical quantification of the dielectrophoretic response of DNA

A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 μL volume. The method has been applied to the character...

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Detalles Bibliográficos
Autores principales: Henning, Anja, Henkel, Jörg, Bier, Frank F, Hölzel, Ralph
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666632/
https://www.ncbi.nlm.nih.gov/pubmed/19351426
http://dx.doi.org/10.1186/1757-5036-1-4
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author Henning, Anja
Henkel, Jörg
Bier, Frank F
Hölzel, Ralph
author_facet Henning, Anja
Henkel, Jörg
Bier, Frank F
Hölzel, Ralph
author_sort Henning, Anja
collection PubMed
description A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 μL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible. PACS: 87.50.ch, 87.80.Fe, 87.85.fK
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spelling pubmed-26666322009-04-08 Label-free electrical quantification of the dielectrophoretic response of DNA Henning, Anja Henkel, Jörg Bier, Frank F Hölzel, Ralph PMC Biophys Research Article A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 μL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible. PACS: 87.50.ch, 87.80.Fe, 87.85.fK BioMed Central 2008-11-05 /pmc/articles/PMC2666632/ /pubmed/19351426 http://dx.doi.org/10.1186/1757-5036-1-4 Text en Copyright © 2008 Henning et al. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Henning, Anja
Henkel, Jörg
Bier, Frank F
Hölzel, Ralph
Label-free electrical quantification of the dielectrophoretic response of DNA
title Label-free electrical quantification of the dielectrophoretic response of DNA
title_full Label-free electrical quantification of the dielectrophoretic response of DNA
title_fullStr Label-free electrical quantification of the dielectrophoretic response of DNA
title_full_unstemmed Label-free electrical quantification of the dielectrophoretic response of DNA
title_short Label-free electrical quantification of the dielectrophoretic response of DNA
title_sort label-free electrical quantification of the dielectrophoretic response of dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666632/
https://www.ncbi.nlm.nih.gov/pubmed/19351426
http://dx.doi.org/10.1186/1757-5036-1-4
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