Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses

The plant-infecting members of the genus Fijivirus (family Reoviridae) have linear dsRNA genomes divided into 10 segments, two of which contain two substantial and non-overlapping ORFs, while the remaining eight are apparently monocistronic. However, one of these – namely segment 5 – contains a seco...

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Detalles Bibliográficos
Autores principales: Firth, Andrew E, Atkins, John F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666654/
https://www.ncbi.nlm.nih.gov/pubmed/19292925
http://dx.doi.org/10.1186/1743-422X-6-32
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author Firth, Andrew E
Atkins, John F
author_facet Firth, Andrew E
Atkins, John F
author_sort Firth, Andrew E
collection PubMed
description The plant-infecting members of the genus Fijivirus (family Reoviridae) have linear dsRNA genomes divided into 10 segments, two of which contain two substantial and non-overlapping ORFs, while the remaining eight are apparently monocistronic. However, one of these – namely segment 5 – contains a second long ORF (~200+ codons) that overlaps the 3' end of the major ORF (~920–940 codons) in the +1 reading frame. In this report, we use bioinformatic techniques to analyze the pattern of base variations across an alignment of fijivirus segment 5 sequences, and show that this 3' ORF has a strong coding signature. Possible translation mechanisms for this unusually positioned ORF are discussed.
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spelling pubmed-26666542009-04-08 Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses Firth, Andrew E Atkins, John F Virol J Short Report The plant-infecting members of the genus Fijivirus (family Reoviridae) have linear dsRNA genomes divided into 10 segments, two of which contain two substantial and non-overlapping ORFs, while the remaining eight are apparently monocistronic. However, one of these – namely segment 5 – contains a second long ORF (~200+ codons) that overlaps the 3' end of the major ORF (~920–940 codons) in the +1 reading frame. In this report, we use bioinformatic techniques to analyze the pattern of base variations across an alignment of fijivirus segment 5 sequences, and show that this 3' ORF has a strong coding signature. Possible translation mechanisms for this unusually positioned ORF are discussed. BioMed Central 2009-03-17 /pmc/articles/PMC2666654/ /pubmed/19292925 http://dx.doi.org/10.1186/1743-422X-6-32 Text en Copyright © 2009 Firth and Atkins; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Firth, Andrew E
Atkins, John F
Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title_full Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title_fullStr Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title_full_unstemmed Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title_short Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses
title_sort analysis of the coding potential of the partially overlapping 3' orf in segment 5 of the plant fijiviruses
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666654/
https://www.ncbi.nlm.nih.gov/pubmed/19292925
http://dx.doi.org/10.1186/1743-422X-6-32
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AT atkinsjohnf analysisofthecodingpotentialofthepartiallyoverlapping3orfinsegment5oftheplantfijiviruses

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