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Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis
BACKGROUND: Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinig...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666751/ https://www.ncbi.nlm.nih.gov/pubmed/19284528 http://dx.doi.org/10.1186/1756-0500-2-33 |
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author | Tazumi, Akihiro Ono, Shinji Sekizuka, Tsuyoshi Moore, John E Millar, B Cherie Matsuda, Motoo |
author_facet | Tazumi, Akihiro Ono, Shinji Sekizuka, Tsuyoshi Moore, John E Millar, B Cherie Matsuda, Motoo |
author_sort | Tazumi, Akihiro |
collection | PubMed |
description | BACKGROUND: Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinigenitalis (UCD-1(T), UK-1 and UK-2). FINDINGS: Following TA cloning and sequencing, the three isolates of T. asinigenitalis were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1(T )and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA(Ile)-tRNA(Ala)-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of boxB and boxA were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of T. asinigenitalis. This was also confirmed by Southern hybridization procedure. CONCLUSION: The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for T. asinigenitalis, which may aid in the phylogenetic positioning of T. asinigenitalis within the genus Taylorella, and in the molecular discrimination of T. asinigenitalis. |
format | Text |
id | pubmed-2666751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26667512009-04-08 Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis Tazumi, Akihiro Ono, Shinji Sekizuka, Tsuyoshi Moore, John E Millar, B Cherie Matsuda, Motoo BMC Res Notes Short Report BACKGROUND: Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinigenitalis (UCD-1(T), UK-1 and UK-2). FINDINGS: Following TA cloning and sequencing, the three isolates of T. asinigenitalis were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1(T )and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA(Ile)-tRNA(Ala)-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of boxB and boxA were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of T. asinigenitalis. This was also confirmed by Southern hybridization procedure. CONCLUSION: The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for T. asinigenitalis, which may aid in the phylogenetic positioning of T. asinigenitalis within the genus Taylorella, and in the molecular discrimination of T. asinigenitalis. BioMed Central 2009-03-03 /pmc/articles/PMC2666751/ /pubmed/19284528 http://dx.doi.org/10.1186/1756-0500-2-33 Text en Copyright © 2008 Matsuda et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Tazumi, Akihiro Ono, Shinji Sekizuka, Tsuyoshi Moore, John E Millar, B Cherie Matsuda, Motoo Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title_full | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title_fullStr | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title_full_unstemmed | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title_short | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis |
title_sort | molecular characterization of the sequences of the 16s-23s rdna internal spacer region (isr) from isolates of taylorella asinigenitalis |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666751/ https://www.ncbi.nlm.nih.gov/pubmed/19284528 http://dx.doi.org/10.1186/1756-0500-2-33 |
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