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In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair

BACKGROUND: Barrier materials as cellulose membranes are used for guided tissue repair. However, it is essential that the surrounding tissues accept the device. The present study histologically evaluated tissue reaction to a microbial cellulose membrane after subcutaneous implantation in mice. Furth...

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Autores principales: Mendes, Péricles Nóbrega, Rahal, Sheila Canevese, Pereira-Junior, Oduvaldo Câmara Marques, Fabris, Viciany Erique, Lenharo, Sara Lais Rahal, de Lima-Neto, João Ferreira, da Cruz Landim-Alvarenga, Fernanda
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667521/
https://www.ncbi.nlm.nih.gov/pubmed/19317903
http://dx.doi.org/10.1186/1751-0147-51-12
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author Mendes, Péricles Nóbrega
Rahal, Sheila Canevese
Pereira-Junior, Oduvaldo Câmara Marques
Fabris, Viciany Erique
Lenharo, Sara Lais Rahal
de Lima-Neto, João Ferreira
da Cruz Landim-Alvarenga, Fernanda
author_facet Mendes, Péricles Nóbrega
Rahal, Sheila Canevese
Pereira-Junior, Oduvaldo Câmara Marques
Fabris, Viciany Erique
Lenharo, Sara Lais Rahal
de Lima-Neto, João Ferreira
da Cruz Landim-Alvarenga, Fernanda
author_sort Mendes, Péricles Nóbrega
collection PubMed
description BACKGROUND: Barrier materials as cellulose membranes are used for guided tissue repair. However, it is essential that the surrounding tissues accept the device. The present study histologically evaluated tissue reaction to a microbial cellulose membrane after subcutaneous implantation in mice. Furthermore, the interaction between mesenchymal stem cells and the biomaterial was studied in vitro to evaluate its ability to act as cellular scaffold for tissue engineering. METHODS: Twenty-five Swiss Albino mice were used. A 10 × 10 mm cellulose membrane obtained through biosynthesis using Acetobacter xylinum bacteria was implanted into the lumbar subcutaneous tissue of each mouse. The mice were euthanatized at seven, 15, 30, 60, and 90 days, and the membrane and surrounding tissues were collected and examined by histology. RESULTS: A mild inflammatory response without foreign body reaction was observed until 30 days post-surgery around the implanted membrane. Polarized microscopy revealed that the membrane remained intact at all evaluation points. Scanning electron microscopy of the cellulose membrane surface showed absence of pores. The in vitro evaluation of the interaction between cells and biomaterial was performed through viability staining analysis of the cells over the biomaterial, which showed that 95% of the mesenchymal stem cells aggregating to the cellulose membrane were alive and that 5% were necrotic. Scanning electron microscopy showed mesenchymal stem cells with normal morphology and attached to the cellulose membrane surface. CONCLUSION: The microbial cellulose membrane evaluated was found to be nonresorbable, induced a mild inflammatory response and may prove useful as a scaffold for mesenchymal stem cells.
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spelling pubmed-26675212009-04-10 In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair Mendes, Péricles Nóbrega Rahal, Sheila Canevese Pereira-Junior, Oduvaldo Câmara Marques Fabris, Viciany Erique Lenharo, Sara Lais Rahal de Lima-Neto, João Ferreira da Cruz Landim-Alvarenga, Fernanda Acta Vet Scand Research BACKGROUND: Barrier materials as cellulose membranes are used for guided tissue repair. However, it is essential that the surrounding tissues accept the device. The present study histologically evaluated tissue reaction to a microbial cellulose membrane after subcutaneous implantation in mice. Furthermore, the interaction between mesenchymal stem cells and the biomaterial was studied in vitro to evaluate its ability to act as cellular scaffold for tissue engineering. METHODS: Twenty-five Swiss Albino mice were used. A 10 × 10 mm cellulose membrane obtained through biosynthesis using Acetobacter xylinum bacteria was implanted into the lumbar subcutaneous tissue of each mouse. The mice were euthanatized at seven, 15, 30, 60, and 90 days, and the membrane and surrounding tissues were collected and examined by histology. RESULTS: A mild inflammatory response without foreign body reaction was observed until 30 days post-surgery around the implanted membrane. Polarized microscopy revealed that the membrane remained intact at all evaluation points. Scanning electron microscopy of the cellulose membrane surface showed absence of pores. The in vitro evaluation of the interaction between cells and biomaterial was performed through viability staining analysis of the cells over the biomaterial, which showed that 95% of the mesenchymal stem cells aggregating to the cellulose membrane were alive and that 5% were necrotic. Scanning electron microscopy showed mesenchymal stem cells with normal morphology and attached to the cellulose membrane surface. CONCLUSION: The microbial cellulose membrane evaluated was found to be nonresorbable, induced a mild inflammatory response and may prove useful as a scaffold for mesenchymal stem cells. BioMed Central 2009-03-24 /pmc/articles/PMC2667521/ /pubmed/19317903 http://dx.doi.org/10.1186/1751-0147-51-12 Text en Copyright © 2009 Mendes et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mendes, Péricles Nóbrega
Rahal, Sheila Canevese
Pereira-Junior, Oduvaldo Câmara Marques
Fabris, Viciany Erique
Lenharo, Sara Lais Rahal
de Lima-Neto, João Ferreira
da Cruz Landim-Alvarenga, Fernanda
In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title_full In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title_fullStr In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title_full_unstemmed In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title_short In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
title_sort in vivo and in vitro evaluation of an acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667521/
https://www.ncbi.nlm.nih.gov/pubmed/19317903
http://dx.doi.org/10.1186/1751-0147-51-12
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