Digital PCR provides sensitive and absolute calibration for high throughput sequencing

BACKGROUND: Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, ther...

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Detalles Bibliográficos
Autores principales: White, Richard A, Blainey, Paul C, Fan, H Christina, Quake, Stephen R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667538/
https://www.ncbi.nlm.nih.gov/pubmed/19298667
http://dx.doi.org/10.1186/1471-2164-10-116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667538/
https://www.ncbi.nlm.nih.gov/pubmed/19298667
http://dx.doi.org/10.1186/1471-2164-10-116

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