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Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinser...
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Formato: | Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667645/ https://www.ncbi.nlm.nih.gov/pubmed/18657235 http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x |
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author | Chiu, Chi-Chou Li, Hsou-min |
author_facet | Chiu, Chi-Chou Li, Hsou-min |
author_sort | Chiu, Chi-Chou |
collection | PubMed |
description | Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins. |
format | Text |
id | pubmed-2667645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-26676452009-04-28 Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane Chiu, Chi-Chou Li, Hsou-min Plant J Original Articles Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins. Blackwell Publishing Ltd 2008-12 2008-08-20 /pmc/articles/PMC2667645/ /pubmed/18657235 http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x Text en © 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd |
spellingShingle | Original Articles Chiu, Chi-Chou Li, Hsou-min Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title | Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title_full | Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title_fullStr | Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title_full_unstemmed | Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title_short | Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
title_sort | tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667645/ https://www.ncbi.nlm.nih.gov/pubmed/18657235 http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x |
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