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Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane

Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinser...

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Detalles Bibliográficos
Autores principales: Chiu, Chi-Chou, Li, Hsou-min
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667645/
https://www.ncbi.nlm.nih.gov/pubmed/18657235
http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x
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author Chiu, Chi-Chou
Li, Hsou-min
author_facet Chiu, Chi-Chou
Li, Hsou-min
author_sort Chiu, Chi-Chou
collection PubMed
description Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins.
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spelling pubmed-26676452009-04-28 Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane Chiu, Chi-Chou Li, Hsou-min Plant J Original Articles Chloroplast inner-membrane proteins Tic40 and Tic110 are first imported from the cytosol into the chloroplast stroma, and subsequently reinserted from the stroma into the inner membrane. However, the mechanism of reinsertion remains unclear. Here we show that Tic40 itself is involved in this reinsertion process. When precursors of either Tic40 or a Tic110 C-terminal truncate, tpTic110-Tic110N, were imported into chloroplasts isolated from a tic40-null mutant, soluble Tic40 and Tic110N intermediates accumulated in the stroma of tic40-mutant chloroplasts, due to a slower rate of reinsertion. We further show that a larger quantity of soluble Tic21 intermediates also accumulated in the stroma of tic40-mutant chloroplasts. In contrast, inner-membrane insertion of the triose-phosphate/phosphate translocator was not affected by the tic40 mutation. Our data suggest that multiple pathways exist for the insertion of chloroplast inner-membrane proteins. Blackwell Publishing Ltd 2008-12 2008-08-20 /pmc/articles/PMC2667645/ /pubmed/18657235 http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x Text en © 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
spellingShingle Original Articles
Chiu, Chi-Chou
Li, Hsou-min
Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title_full Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title_fullStr Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title_full_unstemmed Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title_short Tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
title_sort tic40 is important for reinsertion of proteins from the chloroplast stroma into the inner membrane
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667645/
https://www.ncbi.nlm.nih.gov/pubmed/18657235
http://dx.doi.org/10.1111/j.1365-313X.2008.03638.x
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