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Small-scale, semi-automated purification of eukaryotic proteins for structure determination

A simple approach that allows cost-effective automated purification of recombinant proteins in levels sufficient for functional characterization or structural studies is described. Studies with four human stem cell proteins, an engineered version of green fluorescent protein, and other proteins are...

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Detalles Bibliográficos
Autores principales: Frederick, Ronnie O., Bergeman, Lai, Blommel, Paul G., Bailey, Lucas J., McCoy, Jason G., Song, Jikui, Meske, Louise, Bingman, Craig A., Riters, Megan, Dillon, Nicholas A., Kunert, John, Yoon, Jung Whan, Lim, Ahyoung, Cassidy, Michael, Bunge, Jason, Aceti, David J., Primm, John G., Markley, John L., Phillips, George N., Fox, Brian G.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2668602/
https://www.ncbi.nlm.nih.gov/pubmed/17985212
http://dx.doi.org/10.1007/s10969-007-9032-5