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Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes
BACKGROUND: Antigen-based (as opposed to whole organism) vaccines are actively being pursued for numerous indications. Even though different formulations may produce similar levels of total antigen-specific antibody, the composition of the antibody response can be quite distinct resulting in differe...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2668798/ https://www.ncbi.nlm.nih.gov/pubmed/19390580 http://dx.doi.org/10.1371/journal.pone.0005309 |
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author | Denisova, Galina Denisov, Dimitri Evelegh, Carole Weissgram, Michaela Beck, Jochen Foley, Stephen Ronan Bramson, Jonathan Lorne |
author_facet | Denisova, Galina Denisov, Dimitri Evelegh, Carole Weissgram, Michaela Beck, Jochen Foley, Stephen Ronan Bramson, Jonathan Lorne |
author_sort | Denisova, Galina |
collection | PubMed |
description | BACKGROUND: Antigen-based (as opposed to whole organism) vaccines are actively being pursued for numerous indications. Even though different formulations may produce similar levels of total antigen-specific antibody, the composition of the antibody response can be quite distinct resulting in different levels of therapeutic activity. METHODOLOGY/PRINCIPAL FINDINGS: Using plasmid-based immunization against the proto-oncogene HER-2 as a model, we have demonstrated that affinity-selected epitope mimetics (mimotopes) can provide a defined signature of a polyclonal antibody response. Further, using novel computer algorithms that we have developed, these mimotopes can be used to predict epitope targets. CONCLUSIONS/SIGNIFICANCE: By combining our novel strategy with existing methods of epitope prediction based on physical properties of an individual protein, we believe that this method offers a robust method for characterizing the breadth of epitope-specificity within a specific polyserum. This strategy is useful as a tool for monitoring immunity following vaccination and can also be used to define relevant epitopes for the creation of novel vaccines. |
format | Text |
id | pubmed-2668798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26687982009-04-23 Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes Denisova, Galina Denisov, Dimitri Evelegh, Carole Weissgram, Michaela Beck, Jochen Foley, Stephen Ronan Bramson, Jonathan Lorne PLoS One Research Article BACKGROUND: Antigen-based (as opposed to whole organism) vaccines are actively being pursued for numerous indications. Even though different formulations may produce similar levels of total antigen-specific antibody, the composition of the antibody response can be quite distinct resulting in different levels of therapeutic activity. METHODOLOGY/PRINCIPAL FINDINGS: Using plasmid-based immunization against the proto-oncogene HER-2 as a model, we have demonstrated that affinity-selected epitope mimetics (mimotopes) can provide a defined signature of a polyclonal antibody response. Further, using novel computer algorithms that we have developed, these mimotopes can be used to predict epitope targets. CONCLUSIONS/SIGNIFICANCE: By combining our novel strategy with existing methods of epitope prediction based on physical properties of an individual protein, we believe that this method offers a robust method for characterizing the breadth of epitope-specificity within a specific polyserum. This strategy is useful as a tool for monitoring immunity following vaccination and can also be used to define relevant epitopes for the creation of novel vaccines. Public Library of Science 2009-04-23 /pmc/articles/PMC2668798/ /pubmed/19390580 http://dx.doi.org/10.1371/journal.pone.0005309 Text en Denisova et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Denisova, Galina Denisov, Dimitri Evelegh, Carole Weissgram, Michaela Beck, Jochen Foley, Stephen Ronan Bramson, Jonathan Lorne Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title | Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title_full | Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title_fullStr | Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title_full_unstemmed | Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title_short | Characterizing Complex Polysera Produced by Antigen-Specific Immunization through the Use of Affinity-Selected Mimotopes |
title_sort | characterizing complex polysera produced by antigen-specific immunization through the use of affinity-selected mimotopes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2668798/ https://www.ncbi.nlm.nih.gov/pubmed/19390580 http://dx.doi.org/10.1371/journal.pone.0005309 |
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