The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development

Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transc...

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Autores principales: Exner, Vivien, Aichinger, Ernst, Shu, Huan, Wildhaber, Thomas, Alfarano, Pietro, Caflisch, Amedeo, Gruissem, Wilhelm, Köhler, Claudia, Hennig, Lars
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670505/
https://www.ncbi.nlm.nih.gov/pubmed/19399177
http://dx.doi.org/10.1371/journal.pone.0005335
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author Exner, Vivien
Aichinger, Ernst
Shu, Huan
Wildhaber, Thomas
Alfarano, Pietro
Caflisch, Amedeo
Gruissem, Wilhelm
Köhler, Claudia
Hennig, Lars
author_facet Exner, Vivien
Aichinger, Ernst
Shu, Huan
Wildhaber, Thomas
Alfarano, Pietro
Caflisch, Amedeo
Gruissem, Wilhelm
Köhler, Claudia
Hennig, Lars
author_sort Exner, Vivien
collection PubMed
description Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transcriptional repression in animals, but in plants PRC1-like activities have remained elusive. One candidate protein that could be involved in PRC1-like functions in plants is LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), because LHP1 associates with genes marked by H3K27me3 in vivo and has a chromodomain that binds H3K27me3 in vitro. Here, we show that disruption of the chromodomain of Arabidopsis thaliana LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants. Therefore, binding to H3K27me3 is essential for LHP1 protein function.
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spelling pubmed-26705052009-04-28 The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development Exner, Vivien Aichinger, Ernst Shu, Huan Wildhaber, Thomas Alfarano, Pietro Caflisch, Amedeo Gruissem, Wilhelm Köhler, Claudia Hennig, Lars PLoS One Research Article Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transcriptional repression in animals, but in plants PRC1-like activities have remained elusive. One candidate protein that could be involved in PRC1-like functions in plants is LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), because LHP1 associates with genes marked by H3K27me3 in vivo and has a chromodomain that binds H3K27me3 in vitro. Here, we show that disruption of the chromodomain of Arabidopsis thaliana LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants. Therefore, binding to H3K27me3 is essential for LHP1 protein function. Public Library of Science 2009-04-28 /pmc/articles/PMC2670505/ /pubmed/19399177 http://dx.doi.org/10.1371/journal.pone.0005335 Text en Exner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Exner, Vivien
Aichinger, Ernst
Shu, Huan
Wildhaber, Thomas
Alfarano, Pietro
Caflisch, Amedeo
Gruissem, Wilhelm
Köhler, Claudia
Hennig, Lars
The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title_full The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title_fullStr The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title_full_unstemmed The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title_short The Chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 Is Essential for H3K27me3 Binding and Function during Arabidopsis Development
title_sort chromodomain of like heterochromatin protein 1 is essential for h3k27me3 binding and function during arabidopsis development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670505/
https://www.ncbi.nlm.nih.gov/pubmed/19399177
http://dx.doi.org/10.1371/journal.pone.0005335
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