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Direct Measurement of the Ionization State of an Essential Guanine in the Hairpin Ribozyme
Active site guanines are critical for self-cleavage reactions of several ribozymes, but their precise function(s) in catalysis are unclear. To learn whether protonated or deprotonated forms of guanine predominate in the active site, microscopic pK(a) values were determined for ionization of 8-azagua...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670934/ https://www.ncbi.nlm.nih.gov/pubmed/19330013 http://dx.doi.org/10.1038/nchembio.156 |